Human A Disintegrin And Metalloprotease 10 (ADAM10) RTU ELISA Kit | ADAM10 rtu elisa kit

Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit

Cat. Num. AAA457369

• Gene Names: ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• Reactivity: Human
• Synonyms: A Disintegrin And Metalloprotease 10 (ADAM10); Human A Disintegrin And Metalloprotease 10 (ADAM10) ELISA Kit; CD156-C; CD156c; MADM; Kuz; CDw156; Kuzbanian protein homolog; Mammalian disintegrin-metalloprotease; ADAM10 rtu elisa kit

• Reactivity: Human
• Sequence Length: 748

• Assay Type: Sandwich
• Samples: Serum, Plasma, Tissue Homogenates or Other Biological Fluids.
• Detection Range: 62.5-4000pg/mL
• Sensitivity: 24.6pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM10 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to ADAM10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ADAM10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ADAM10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557251
• NCBI GeneID: 102
• NCBI Accession #: NP_001101.1
• NCBI GenBank Nucleotide #: NM_001110.3
• UniProt Accession #: O14672; Q10742; Q92650; B4DU28
• Molecular Weight: 49,047 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 10 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 10
• NCBI Official Symbol: ADAM10
• NCBI Official Synonym Symbols: RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 10
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 10
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM10
• UniProt Synonym Gene Names: KUZ; MADM; ADAM 10

NCBI Description

Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. Alternate splicing results in multiple transcript variants encoding different proteins that may undergo similar processing. [provided by RefSeq, Feb 2016]

Uniprot Description

Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface (PubMed:20592283). Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP) (PubMed:26686862, PubMed:11786905). Contributes to the normal cleavage of the cellular prion protein (PubMed:11477090). Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity (PubMed:12475894). Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis (). Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form (PubMed:17557115). Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B (PubMed:19114711, PubMed:21288900). May regulate the EFNA5-EPHA3 signaling (PubMed:16239146).