Integrin alpha-M Recombinant Antibody | ITGAM recombinant antibody
Anti-Integrin alpha-M [107]
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
Integrin alpha-M; Anti-Integrin alpha-M [107]; CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD11b; complement receptor 3; ITGAM recombinant antibody
Reactivity
Human
Isotype
Mouse IgG1, kappa
Clone Number
107
Specificity
This antibody binds to the MIDAS face of the A domain from integrin CD11b.
Purity/Purification
Protein A Affinity Purified
Sequence Length
1152
Applicable Applications for ITGAM recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
The antibody binds specifically to integrin alpha-M, a protein subunit of the alpha-M- beta-2 molecule, which is expressed on the surface of leukocytes. Integrin alpha-M is involved in the adhesive interaction between molecules of the innate immune system, and plays a role in the uptake of complement-coated particles. The monoclonal antibody 107 has a blocking activity by binding to the metal-ion-dependent-adhesion site, favouring a low affinity conformation of the antigen.
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
Wild type (WT) recombinant CD11b.
Chimeric Use Statement
This chimeric mouse antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for ITGAM recombinant antibody
Recombinant monoclonal antibody to Integrin alpha-M. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 107. This chimeric mouse antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for ITGAM recombinant antibody
References
Li R, Haruta I, Rieu P, Sugimori T, Xiong JP, Arnaout MA. Characterization of a conformationally sensitive murine monoclonal antibody directed to the metal ion-dependent adhesion site face of integrin CD11b. J Immunol. 2002 Feb 1; 168(3):1219-25. PMID:11801658
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
127,307 Da
NCBI Official Full Name
Integrin alpha-M
NCBI Official Synonym Full Names
integrin subunit alpha M
NCBI Official Symbol
ITGAM
NCBI Official Synonym Symbols
CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
NCBI Protein Information
integrin alpha-M
UniProt Protein Name
Integrin alpha-M
Protein Family
UniProt Gene Name
ITGAM
UniProt Synonym Gene Names
CD11B; CR3A
UniProt Entry Name
ITAM_HUMAN
NCBI Description
This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
Research Articles on ITGAM
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Product Notes
The ITGAM itgam (Catalog #AAA488201) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Integrin alpha-M [107] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Integrin alpha-M can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). The antibody binds specifically to integrin alpha-M, a protein subunit of the alpha-M- beta-2 molecule, which is expressed on the surface of leukocytes. Integrin alpha-M is involved in the adhesive interaction between molecules of the innate immune system, and plays a role in the uptake of complement-coated particles. The monoclonal antibody 107 has a blocking activity by binding to the metal-ion-dependent-adhesion site, favouring a low affinity conformation of the antigen. Researchers should empirically determine the suitability of the ITGAM itgam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Integrin alpha-M, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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