Immunofluorescence (IF)
( Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-C3a-receptor antibody 3G7 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
C3a-receptor Recombinant Antibody | C3AR1 recombinant antibody
Anti-C3a-receptor [3G7]
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
C3a-receptor; Anti-C3a-receptor [3G7]; Complement component receptor 1; C3AR1; C3AR1 recombinant antibody
Reactivity
Human
Isotype
Rabbit IgG, kappa
Clone Number
3G7
Specificity
Binds specifically to the 2e loop of the human C3a receptor, residues 185-193.
Purity/Purification
Protein A Affinity Purified
Sequence Length
475
Applicable Applications for C3AR1 recombinant antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
This scFv antibody binds to the 2e loop of the human C3a receptor, a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release.
Buffer Composition
PBS only with 0.02% Proclin 300.
Immunogen
The 2e loop of the C3a receptor.
Chimeric Use Statement
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
( Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-C3a-receptor antibody 3G7 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
( Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-C3a-receptor antibody 3G7 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 3G7 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for C3AR1 recombinant antibody
Recombinant monoclonal antibody to C3a-receptor. Manufactured using a Recombinant Platform with variable regions (i.e. specificity) from the scFv 3G7. This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Mouse scFv format, for improved compatibility with existing reagents, assays and techniques.
Product Categories/Family for C3AR1 recombinant antibody
References
Hawlisch et al. 2001 Site-specific anti-C3a receptor single-chain antibodies selectied by differential panning on cellulose sheets. Analytica Biochemistry 2001; 293(1):142-145 PMID:11373092
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
49,755 Da
NCBI Official Full Name
C3a receptor
UniProt Protein Name
C3a anaphylatoxin chemotactic receptor
Protein Family
UniProt Gene Name
C3AR1
UniProt Synonym Gene Names
C3AR; C3a-R
UniProt Entry Name
C3AR_CAVPO
Uniprot Description
Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.
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Product Notes
The C3a-receptor c3ar1 (Catalog #AAA488188) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-C3a-receptor [3G7] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's C3a-receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). This scFv antibody binds to the 2e loop of the human C3a receptor, a G protein-coupled receptor stimulating chemotaxis, superoxide anion generation and granule enzyme release. Researchers should empirically determine the suitability of the C3a-receptor c3ar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C3a-receptor, Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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