Ferritin L-chain, Western Blot Positive Control Protein
Ferritin L-chain, Human, Western Blot Positive Control (FTL)
Applications
Western Blot
Purity
Highly PurifiedHighly purified (95%)
Synonyms
Ferritin L-chain; Western Blot Positive Control; Human; Western Blot Positive Control (FTL); Western Blot Positive Control protein
Host
Human
Specificity
Recombinant Human Ferritin L-chain
Purity/Purification
Highly Purified
Highly purified (95%)
Highly purified (95%)
Form/Format
Supplied as a liquid in denaturing SDS-PAGE sample buffer r ready to load on gels.
Applicable Applications for Ferritin L-chain, Western Blot Positive Control protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 10ul/lane (ready to use)
Dilution: Western Blot: 10ul/lane (ready to use)
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Ferritin L-chain, Western Blot Positive Control protein
Western Blot positive control for F4015-65 (antiserum) and F4015-70 (affinity purified).
Elemental iron is required for a variety of normal cellular functions. It is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species. The heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains. 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3. FTH subunit (rich in human heart) is located on chromosome 11. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy. Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation. L-chains assist in core formation.
Elemental iron is required for a variety of normal cellular functions. It is vital for proper growth and development. Ferritin is the major protein involved in iron sequestration and detoxification. Ferritin is found in all living species. Mammalian liver and spleen ferritin (~450kD) consists of 24 subunits of 2 species. The heavy subunit (~21kD; FTH) and the light subunit (~19kD; FTL). The 2 types of apoferritin subunits were designated H and L for heart and liver, respectively. Ferritin molecules from plants and bacteria contain only H-type chains. 'H-type' is associated with the presence of centers catalyzing the oxidation of two Fe(II) atoms. FTL subunit (rich in human liver and spleen) is coded by a gene in segment 19q13.3. FTH subunit (rich in human heart) is located on chromosome 11. Ferritin is capable of storing up to 4500 atoms of ferric iron. The H-to-L ratio within ferritin varies in a tissue-specific manner. This ratio is also influenced by pathophysiological conditions, including inflammation and malignancy. Hyperferritinemia-cataract syndrome has a mutation in the iron response element (IRE) in the 5-prime noncoding region of the FTL gene. Synthesis of both ferritin subunits is controlled by a common cytosolic protein, iron regulatory proteins (IRPs), which binds to the iron-responsive element (IRE) in the 5'-UTR of the H- and L-ferritin mRNAs. H-chains are important for Fe(II) oxidation. L-chains assist in core formation.
Product Categories/Family for Ferritin L-chain, Western Blot Positive Control protein
Similar Products
Product Notes
The Ferritin L-chain, Western Blot Positive Control (Catalog #AAA634724) is a Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ferritin L-chain, Western Blot Positive Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 10ul/lane (ready to use). Researchers should empirically determine the suitability of the Ferritin L-chain, Western Blot Positive Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ferritin L-chain, Western Blot Positive Control, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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