Bacterial/Permeability-Increasing Protein | BPI protein

Bacterial / Permeability-Increasing Protein, Human Neutrophil (BPI, CAP57)

Cat. Num. AAA844054

• Gene Names: BPI; rBPI; BPIFD1
• Applications: SDS-Page
• Purity: >=95% by SDS-PAGE
• Synonyms: Bacterial/Permeability-Increasing Protein; Bacterial / Permeability-Increasing Protein; Human Neutrophil (BPI; CAP57); BPI; CAP57; BPI protein

• Host: Human Neutrophil
• Purity/Purification: >=95% by SDS-PAGE
• Form/Format: Frozen in 80 mM Citrate Phosphate, pH 5.6, 0.75 M NaCl.
• Sequence Length: 487

• Applicable Applications for BPI protein: SDS-PAGE
• Source: Human Whole Blood. Shown to be non-reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA approved tests.
• Extinction Coefficient: 1.00
• Appearance: Frozen
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: Aliquot and store at -70°C or lower. Avoid repeated freezing and thawing cycles.; Shipping: Dry Ice.

Testing Data

Related Product Information for BPI protein

Background: Bactericidal/permeability increasing protein (BPI) is a 456 residue protein which is part of the innate immune system. BPI was initially identified in neutrophils, but is found in other tissues including the epithelial lining of mucus membranes. It is an endogenous antibiotic protein with potent killing activity against Gram-negative bacteria. It binds to compounds called lipopolysaccharides produced by Gram-negative bacteria. Lipolysaccharides are potent activators of the immune system; however BPI at certain concentrations can prevent this activation. Bacterial/Permeability-Increasing Protein (BPI) is present in the azurophilic granules of polymorphonuclear leukocytes (PMN). BPI is toxic only toward Gram-negative bacteria. This specificity is attributable to the strong attraction of BPI for the lipopolysaccharides (LPS) in the bacterial envelope. BPI is also an important antigen for anti-neutrophil cytoplasmic autoantibodies (ANCA) in vasculitis.

Product Categories/Family for BPI protein

Proteins and Enzymes; Native Proteins (NEW CATEGORY) Proteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 157276599
• NCBI GeneID: 671
• NCBI Accession #: NP_001716.2
• NCBI GenBank Nucleotide #: NM_001725.2
• UniProt Accession #: P17213; Q1ZZU8; Q5JRW0; Q8IW58; Q9BYZ9; Q9H1L2; Q9H1M8; Q9H203; Q9UCT4; Q9UD65; B2RCY2
• Molecular Weight: 55 kDa
• NCBI Official Full Name: bactericidal permeability-increasing protein
• NCBI Official Synonym Full Names: bactericidal/permeability-increasing protein
• NCBI Official Symbol: BPI
• NCBI Official Synonym Symbols: rBPI; BPIFD1
• NCBI Protein Information: bactericidal permeability-increasing protein
• UniProt Protein Name: Bactericidal permeability-increasing protein
• Protein Family: Bactericidal permeability-increasing protein
• UniProt Gene Name: BPI
• UniProt Synonym Gene Names: BPI
• UniProt Entry Name: BPI_HUMAN

NCBI Description

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisms. [provided by RefSeq, Nov 2014]

Uniprot Description

BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Secreted, signal peptide; Secreted; Cell surface

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: integral to plasma membrane; cytoplasm

Molecular Function: lipopolysaccharide binding

Biological Process: negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; immune response; negative regulation of tumor necrosis factor production; negative regulation of macrophage activation; defense response to Gram-negative bacterium