Human Bactericidal permeability-increasing protein ELISA Kit | BPI elisa kit

Human Bactericidal permeability-increasing protein ELISA Kit

Cat. Num. AAA765828

• Gene Names: BPI; rBPI; BPIFD1
• Reactivity: Human
• Synonyms: Bactericidal permeability-increasing protein; Human Bactericidal permeability-increasing protein ELISA Kit; BPI (Bactericidal/Permeability Increasing Protein)/Bactericidal permeability-increasing protein/CAP 57; BPI elisa kit

• Reactivity: Human
• Sequence Length: 487

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for BPI elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human BPI antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human BPI antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human BPI amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human BPI can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 157276599
• NCBI GeneID: 671
• NCBI Accession #: NP_001716.2
• NCBI GenBank Nucleotide #: NM_001725.2
• Molecular Weight: 53,900 Da
• NCBI Official Full Name: bactericidal permeability-increasing protein
• NCBI Official Synonym Full Names: bactericidal/permeability-increasing protein
• NCBI Official Symbol: BPI
• NCBI Official Synonym Symbols: rBPI; BPIFD1
• NCBI Protein Information: bactericidal permeability-increasing protein
• UniProt Protein Name: Bactericidal permeability-increasing protein
• Protein Family: Bactericidal permeability-increasing protein
• UniProt Gene Name: BPI
• UniProt Synonym Gene Names: BPI
• UniProt Entry Name: BPI_HUMAN

NCBI Description

This gene encodes a lipopolysaccharide binding protein. It is associated with human neutrophil granules and has antimicrobial activity against gram-negative organisms. [provided by RefSeq, Nov 2014]

Uniprot Description

BPI: The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Belongs to the BPI/LBP/Plunc superfamily. BPI/LBP family.

Protein type: Cell surface; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: cytoplasm; extracellular space; integral to plasma membrane

Molecular Function: lipopolysaccharide binding

Biological Process: defense response to Gram-negative bacterium; immune response; negative regulation of interleukin-6 production; negative regulation of interleukin-8 production; negative regulation of macrophage activation; negative regulation of tumor necrosis factor production