Troponin T Native Protein | TNNT2 native protein

Highly pure native Human Troponin T protein

Cat. Num. AAA5308441

• Gene Names: TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• Purity: > 95% pure
• Synonyms: Troponin T; Highly pure native Human Troponin T protein; Troponin T protein; TNNT2 native protein

• Host: Human
• Purity/Purification: > 95% pure
• Form/Format: 50 mM Tris-HCl, pH 7.2 containing 6M Urea, 250 mM NaCl, 15 mM beta-mercaptoethanol, 1 mM EDTA. No preservative added.
• Concentration: 0.726 mg/ml protein (varies by lot)
• Sequence Length: 28

• Application Notes: Troponin T is only sparingly soluble at neutral pH and physiological salt concentrations. If removing urea from this product, increase ionic strength to at least 500 mM, and include 10 mM beta-Mercaptoethanol to reduce intermolecular aggregation.
• Source Note: Human cardiac tissue
• Dry Ice Shipment: Extra charge may be applied to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Aliquot and store at -20 degree C. Avoid repeated freeze/thaw cycles.

Product Categories/Family for TNNT2 native protein

Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 547047
• NCBI GeneID: 7139
• Molecular Weight: 30,681 Da
• NCBI Official Full Name: troponin T, partial
• NCBI Official Synonym Full Names: troponin T2, cardiac type
• NCBI Official Symbol: TNNT2
• NCBI Official Synonym Symbols: CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6
• NCBI Protein Information: troponin T, cardiac muscle
• UniProt Protein Name: Troponin T, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNT2
• UniProt Synonym Gene Names: TnTc; cTnT

NCBI Description

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytosol; sarcomere; striated muscle thin filament; troponin complex

Molecular Function: actin binding; protein binding; tropomyosin binding; troponin C binding; troponin I binding

Biological Process: muscle filament sliding; negative regulation of ATPase activity; positive regulation of ATPase activity; regulation of heart contraction; response to calcium ion; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2; Cardiomyopathy, Familial Restrictive, 3