Cardiac Troponin, I Subunit Native Protein | TNNI3 native protein

Human Cardiac Troponin, I Subunit

Cat. Num. AAA686334

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• Applications: Western Blot, ELISA
• Purity: > 95% based on SDS-PAGE; Purified.
• Synonyms: Cardiac Troponin; I Subunit; Human Cardiac Troponin; TNNI3 native protein

• Host: Human
• Purity/Purification: > 95% based on SDS-PAGE; Purified.
• Form/Format: Liquid
• Concentration: >0.2 mg/ml (varies by lot)
• Sequence Length: 210

• Applicable Applications for TNNI3 native protein: Western Blot (WB), ELISA (EIA)
• Conjugate: UnConjugated
• Buffer: 6M Urea, 75 mM Tris, pH 8.0, 15 mM mercaptoethanol, 10mM EDTA
• Country of Origin: Human heart tissue from US donors (donors serum was tested and found negative for HBsAg, HIV-1, HIV-2, and HCV antibodies by FDA approved tests.)
• Preparation and Storage: Store at -20 degree C or colder

Product Categories/Family for TNNI3 native protein

Purified Proteins and Immunoglobulins

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I3, cardiac type
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• NCBI Protein Information: troponin I, cardiac muscle
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytosol; sarcomere; troponin complex

Molecular Function: actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding

Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; muscle filament sliding; negative regulation of ATPase activity; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Dilated, 2a; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Familial Restrictive, 1