PTP1B elisa kit

PTP1B Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500512

• Gene Names: PTPN1; PTP1B
• Reactivity: Human, Mouse, Rat
• Synonyms: PTP1B; PTP1B Colorimetric Cell-Based ELISA Kit; CPTP1; Protein-protein-tyrosine phosphatase HA2; Protein-tyrosine phosphatase 1B; Protein-tyrosine phosphatase non-receptor type 1; PTN1; PTP-1B; PTP-HA2; PTPN1; PTP1B elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 362

• Preparation and Storage: Store at 4 degree C.

Related Product Information for PTP1B elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for PTP1B elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 519666804
• NCBI GeneID: 5770
• NCBI Accession #: NP_001265547.1
• NCBI GenBank Nucleotide #: NM_001278618.1
• UniProt Accession #: P18031; Q5TGD8; Q9BQV9; Q9NQQ4
• Molecular Weight: 49967
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: PTPN1
• NCBI Official Synonym Symbols: PTP1B
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• UniProt Gene Name: PTPN1
• UniProt Synonym Gene Names: PTP1B; PTP-1B

NCBI Description

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Phosphatase; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 20q13.13

Cellular Component: cytosol; early endosome; endoplasmic reticulum; plasma membrane

Molecular Function: cadherin binding; enzyme binding; ephrin receptor binding; insulin receptor binding; protein binding; protein kinase binding; protein phosphatase 2A binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; RNA binding; zinc ion binding

Biological Process: actin cytoskeleton reorganization; activation of JNK activity; activation of signaling protein activity involved in unfolded protein response; endoplasmic reticulum unfolded protein response; insulin receptor signaling pathway; IRE1-mediated unfolded protein response; negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; negative regulation of ERK1 and ERK2 cascade; negative regulation of insulin receptor signaling pathway; negative regulation of MAP kinase activity; negative regulation of PERK-mediated unfolded protein response; negative regulation of signal transduction; negative regulation of vascular endothelial growth factor receptor signaling pathway; peptidyl-tyrosine dephosphorylation involved in inactivation of protein kinase activity; positive regulation of IRE1-mediated unfolded protein response; positive regulation of receptor catabolic process; protein amino acid dephosphorylation; regulation of endocytosis; regulation of hepatocyte growth factor receptor signaling pathway; regulation of intracellular protein transport; regulation of signal transduction

Disease: Diabetes Mellitus, Noninsulin-dependent