Rabbit PTP1B Polyclonal Antibody | anti-PTP1B antibody

PTP1B Antibody

Cat. Num. AAA9503781

• Gene Names: PTPN1; PTP1B
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Synonyms: PTP1B; Polyclonal Antibody; PTP1B Antibody; CPTP1; Protein-protein-tyrosine phosphatase HA2; Protein-tyrosine phosphatase 1B; Protein-tyrosine phosphatase; non-receptor type 1; PTN1; PTP-1B; PTP-HA2; PTPN1; anti-PTP1B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: PTP1B Antibody detects endogenous levels of total PTP1B protein.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 435

• Applicable Applications for anti-PTP1B antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Target Modification: Unmodified/Total
• Immunogen: The antiserum was produced against synthesized peptide derived from human PTP1B.
• Immunogen Range: 16-65
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PTP1B Antibody. The picture on the right is blocked with the synthesized peptide.)

Western Blot (WB)

(Western blot analysis of lysates from COS7 cells, treated with UV 30', using PTP1B Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 519666804
• NCBI GeneID: 5770
• NCBI Accession #: NP_001265547.1
• NCBI GenBank Nucleotide #: NM_001278618.1
• UniProt Accession #: P18031; Q5TGD8; Q9BQV9; Q9NQQ4
• Molecular Weight: 49 kDa
• NCBI Official Full Name: tyrosine-protein phosphatase non-receptor type 1 isoform 2
• NCBI Official Synonym Full Names: protein tyrosine phosphatase, non-receptor type 1
• NCBI Official Symbol: PTPN1
• NCBI Official Synonym Symbols: PTP1B
• NCBI Protein Information: tyrosine-protein phosphatase non-receptor type 1
• UniProt Protein Name: Tyrosine-protein phosphatase non-receptor type 1
• UniProt Gene Name: PTPN1
• UniProt Synonym Gene Names: PTP1B; PTP-1B

NCBI Description

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

Uniprot Description

Tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation of EIF2AK3/PERK; inactivating the protein kinase activity of EIF2AK3/PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. May also regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.