PKC theta elisa kit
Phospho-PKC theta (Thr538) Colorimetric Cell-Based ELISA Kit
Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non- phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.
NCBI and Uniprot Product Information
NCBI Description
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. [provided by RefSeq, Jul 2008]
Uniprot Description
PKCT: an AGC kinase of the PKC family. A novel PKC: Ca2+ independent but still regulated by PS, DAG and phorbol esters. Contains 2 zinc-dependent phorbol-ester and DAG binding domains. Expressed in skeletal muscle and hematopoietic cells. May play a role in T cell receptor signaling and insulin resistance. Required for TCR-induced NF-kappaB activation in mature T lymphocytes.
Protein type: AGC group; Delta subfamily; EC 2.7.11.13; Kinase, protein; PKC family; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 10p15.1
Cellular Component: aggresome; cytosol; immunological synapse; intracellular; microtubule organizing center; plasma membrane
Molecular Function: ATP binding; metal ion binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity
Biological Process: axon guidance; Fc-epsilon receptor signaling pathway; inflammatory response; membrane protein ectodomain proteolysis; negative regulation of insulin receptor signaling pathway; peptidyl-serine phosphorylation; platelet activation; positive regulation of interleukin-17 production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-4 production; positive regulation of NF-kappaB transcription factor activity; positive regulation of T cell activation; positive regulation of T cell proliferation; positive regulation of T-helper 17 type immune response; positive regulation of T-helper 2 cell activation; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; regulation of cell growth; regulation of megakaryocyte differentiation; regulation of platelet aggregation; regulation of transcription, DNA-templated; T cell receptor signaling pathway
Research Articles on PKC theta
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Product Notes
The Human, Mouse, Rat PKC theta prkcq (Catalog #AAA9501215) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9501215 ELISA Kit recognizes Human, Mouse, Rat PKC theta. It is sometimes possible for the material contained within the vial of "PKC theta, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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