Nuclear Receptor NR4A1 ELISA Kit | NR4A1 elisa kit

Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500431

• Gene Names: NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• Reactivity: Human, Mouse, Rat
• Synonyms: Nuclear Receptor NR4A1; Nuclear Receptor NR4A1 Colorimetric Cell-Based ELISA Kit; Nuclear receptor subfamily 4 group A member 1; Orphan nuclear receptor HMR; Early response protein NAK1; TR3 orphan receptor; ST-59; NR4A1; GFRP1; HMR; NAK1; NR4A1 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 611

• Preparation and Storage: Store at 4 degree C.

Related Product Information for NR4A1 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for NR4A1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 320202956
• NCBI GeneID: 3164
• NCBI Accession #: NP_001189162.1
• NCBI GenBank Nucleotide #: NM_001202233.1
• UniProt Accession #: P22736; Q15627; Q53Y00; Q6IBU8; B4DML7
• Molecular Weight: 64463
• NCBI Official Full Name: nuclear receptor subfamily 4 group A member 1 isoform 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 4 group A member 1
• NCBI Official Symbol: NR4A1
• NCBI Official Synonym Symbols: HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
• NCBI Protein Information: nuclear receptor subfamily 4 group A member 1
• UniProt Protein Name: Nuclear receptor subfamily 4 group A member 1
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR4A1
• UniProt Synonym Gene Names: GFRP1; HMR; NAK1; Nur77

NCBI Description

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Nur77: an orphan nuclear receptor and immediate-early gene that regulates cellular proliferation, apoptosis, inflammation, and glucose metabolism. Induced by exercise in muscle and is a functional regulator of glucose metabolism in skeletal muscle. Its level decreases in the muscle of obese insulin-resistant men. Acts concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. A mediator of TCR-directed thymocyte apoptosis. TCR-signaling induces a FAIM/Akt/Nur77 signaling pathway that is critical for modulating apoptosis in developing thymocytes. A physiological substrate of the MEK-ERK-RSK cascade that modulates nuclear export and intracellular translocation during T cell death. Binds DNA as a monomer. Interacts with GADD45GIP1. Overexpression of Nur77 induces the expression of both p300 and HDAC1. Acetylation by p300 and HDAC1 may regulate the rapid turnover of Nur77 protein.

Protein type: Apoptosis; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: cytosol; nuclear membrane; nucleoplasm; nucleus

Molecular Function: DNA binding; ligand-dependent nuclear receptor activity; protein binding; protein heterodimerization activity; sequence-specific DNA binding; steroid hormone receptor activity; zinc ion binding

Biological Process: cell migration during sprouting angiogenesis; cellular response to corticotropin-releasing hormone stimulus; cellular response to fibroblast growth factor stimulus; fat cell differentiation; intracellular receptor signaling pathway; negative regulation of cell cycle; positive regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter; protein kinase B signaling; signal transduction; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter