IKK-alpha/beta elisa kit

IKK-alpha/beta Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500308

• Gene Names: CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• Reactivity: Human, Mouse, Rat
• Synonyms: IKK-alpha/beta; IKK-alpha/beta Colorimetric Cell-Based ELISA Kit; CHUK; Conserved helix-loop-helix ubiquitous kinase; I kappa-B kinase alpha; I-kappa-B kinase 1; IkappaB kinase; IkBKA; IKK-A; IKK-alpha; IKK1; IKKA; Inhibitor of nuclear factor kappa-B kinase alpha subunit; kinase IKK-alpha; NFKBIKA; Nuclear factor NFkappaB inhibitor kinase alpha; I-kappa-B kinase 2; I-kappa-B-kinase beta; IkBKB; IKK-B; IKK-beta; IKK2; IKKB; kinase IKK-beta; NFKBIKB; Nuclear factor NF-kappa-B inhibitor kinase beta; IKK-alpha/beta elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 745

• Samples: Researchers plate their cell line of choice
• Assay Type: Non-Phospho Cell-Based ELISA
• Sensitivity: >=5000 cells/well
• Detection Method: Colorimetric 450 nm
• Preparation and Storage: Store at 4 degree C.

Product Categories/Family for IKK-alpha/beta elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 62241001
• NCBI GeneID: 1147
• NCBI Accession #: NP_001269.3
• NCBI GenBank Nucleotide #: NM_001278.4
• UniProt Accession #: O15111; O14666; Q13132; Q5W0I4; Q92467
• Molecular Weight: 84654; 86564
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit alpha isoform 1
• NCBI Official Synonym Full Names: conserved helix-loop-helix ubiquitous kinase
• NCBI Official Symbol: CHUK
• NCBI Official Synonym Symbols: IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit alpha
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit alpha
• UniProt Gene Name: CHUK
• UniProt Synonym Gene Names: IKKA; TCF16; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; IKK1; NFKBIKA; TCF-16

NCBI Description

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. [provided by RefSeq, Jul 2008]

Uniprot Description

IKKA: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B, leading to their dissociation from NF-kappa-B and ultimately to their degradation. Phosphorylated and activated downstream of growth factor receptors, IL1R, and TNFR by NAK, IRAK and NIK, respectively. Preferentially found as a heterodimer with IKK-beta but also as an homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. Heterodimers form the active complex. The tripartite complex can also bind to MAP3K14/NIK, MEKK1, IKAP and IKB-alpha-p65-p50 complex. Inhibitors are under development to treat arthritis, inflammation, and apoptotic aspects of cancer. Aspirin (sodium salicylate) selectively binds IKK and reduces inflammation. Misexpression and inhibitors show involvement in insulin sensitization in obese rodents. Mutations in IKK-gamma are found in several immune deficiencies: hyper IgM syndrome, incontinentia pigmenti [embryonic lethal in males, defects in skin, hair, teeth in females] and hypohidrotic ectodermal dysplasia; recurrent infections and defects in teeth, hair, and sweat glands]. Inhibitors: CHS 828, NBD peptide, BMS-345541.

Protein type: EC 2.7.11.10; IKK family; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 10q24.31

Cellular Component: cytoplasm; cytoplasmic side of plasma membrane; cytosol; IkappaB kinase complex; intracellular membrane-bound organelle; nucleoplasm

Molecular Function: ATP binding; IkappaB kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase activity

Biological Process: anatomical structure morphogenesis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cellular response to cadmium ion; cellular response to reactive oxygen species; cellular response to tumor necrosis factor; Fc-epsilon receptor signaling pathway; I-kappaB kinase/NF-kappaB signaling; I-kappaB phosphorylation; immune response; inflammatory response; innate immune response; MyD88-independent toll-like receptor signaling pathway; negative regulation of NF-kappaB transcription factor activity; NIK/NF-kappaB signaling; nucleotide-binding oligomerization domain containing signaling pathway; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of interferon-alpha secretion; positive regulation of NF-kappaB transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; protein amino acid phosphorylation; protein kinase B signaling; regulation of tumor necrosis factor-mediated signaling pathway; response to acetate; response to amino acid; response to drug; response to hydroperoxide; response to lipopolysaccharide; response to toxin; response to virus; Rho protein signal transduction; skeletal muscle contraction; stimulatory C-type lectin receptor signaling pathway; stress-activated MAPK cascade; striated muscle cell differentiation; T cell receptor signaling pathway; tumor necrosis factor-mediated signaling pathway

Disease: Cocoon Syndrome