HDAC1 elisa kit

HDAC1 Colorimetric Cell-Based ELISA Kit

Cat. Num. AAA9500273

• Gene Names: HDAC1; HD1; RPD3; GON-10; RPD3L1
• Reactivity: Human, Mouse, Rat
• Synonyms: HDAC1; HDAC1 Colorimetric Cell-Based ELISA Kit; HD1; Histone deacetylase 1; RPD3L1; HDAC1 elisa kit

• Reactivity: Human, Mouse, Rat
• Form/Format: One 96-Well Plate
• Sequence Length: 482

• Preparation and Storage: Store at 4 degree C.

Related Product Information for HDAC1 elisa kit

Background/Introduction: The Colorimetric Cell-Based ELISA Kit allows for the detection of various target proteins and the effects that certain stimulation conditions have on target protein expression in different cell lines. Qualitative determination of target protein concentration is achieved by an indirect ELISA format. In essence, the target protein is captured by target-specific primary (1 degree) antibodies while the HRP-conjugated secondary (2 degree) antibodies bind the Fc region of the 1 degree antibody. Through this binding, the HRP enzyme conjugated to the 2 degree antibody can catalyze a colorimetric reaction upon substrate addition. Due to the qualitative nature of the Cell-Based ELISA, multiple normalization methods are described: 1) a monoclonal antibody specific for human GAPDH is included to serve as an internal positive control in normalizing the target absorbance values. 2) Following the colorimetric measurement of HRP activity via substrate addition, the Crystal Violet whole-cell staining method is used to determine cell density. After staining, the results can be analyzed by normalizing the absorbance values to cell amounts, by which the plating difference can be adjusted. 3) If a phosphorylated target is being detected, an antibody against the non-phosphorylated counterpart will be provided for normalization purposes. The absorbance values obtained for the non-phosphorylated target can be used to normalize the absorbance values for the phosphorylated target.

Product Categories/Family for HDAC1 elisa kit

Cell Based ELISA Kit

NCBI and Uniprot Product Information

• NCBI GI #: 13128860
• NCBI GeneID: 3065
• NCBI Accession #: NP_004955.2
• NCBI GenBank Nucleotide #: NM_004964.2
• UniProt Accession #: Q13547; Q92534
• Molecular Weight: 55103
• NCBI Official Full Name: histone deacetylase 1
• NCBI Official Synonym Full Names: histone deacetylase 1
• NCBI Official Symbol: HDAC1
• NCBI Official Synonym Symbols: HD1; RPD3; GON-10; RPD3L1
• NCBI Protein Information: histone deacetylase 1
• UniProt Protein Name: Histone deacetylase 1
• UniProt Gene Name: HDAC1
• UniProt Synonym Gene Names: RPD3L1; HD1

NCBI Description

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

HDAC1: a transcriptional regulator of the histone deacetylase family, subfamily 1. Deacetylates lysine residues on the N-terminal part of the core histones H2A, H2B, H3 AND H4. Plays an important role in transcriptional regulation, cell cycle progression and developmental events.

Protein type: EC 3.5.1.98; Hydrolase; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p35.2-p35.1

Cellular Component: chromatin; cytoplasm; cytosol; histone deacetylase complex; neuron projection; nuclear chromatin; nucleoplasm; nucleus; NuRD complex; perinuclear region of cytoplasm; protein complex; Sin3 complex

Molecular Function: activating transcription factor binding; deacetylase activity; DNA binding transcription factor activity; enzyme binding; histone deacetylase activity; histone deacetylase binding; NAD-dependent histone deacetylase activity (H3-K14 specific); NF-kappaB binding; nucleosomal DNA binding; p53 binding; protein binding; protein complex binding; protein deacetylase activity; protein N-terminus binding; transcription factor binding; transcription regulatory region DNA binding

Biological Process: ATP-dependent chromatin remodeling; beta-catenin-TCF complex assembly; blood coagulation; cellular response to oxidative stress; cellular response to tumor necrosis factor; chromatin organization; chromatin remodeling; circadian regulation of gene expression; embryonic digit morphogenesis; epidermal cell differentiation; histone deacetylation; methylation-dependent chromatin silencing; negative regulation of apoptosis; negative regulation of canonical Wnt signaling pathway; negative regulation of cell proliferation; negative regulation of gene expression; negative regulation of insulin secretion; negative regulation of myotube differentiation; negative regulation of neuron apoptosis; negative regulation of peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; odontogenesis of dentine-containing teeth; positive regulation of cell proliferation; positive regulation of chemokine (C-X-C motif) ligand 2 production; positive regulation of interleukin-1 production; positive regulation of oligodendrocyte differentiation; positive regulation of receptor biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of tumor necrosis factor production; positive regulation of type B pancreatic cell apoptotic process; positive regulation of tyrosine phosphorylation of STAT protein; protein deacetylation; regulation of megakaryocyte differentiation; response to amphetamine; response to caffeine; response to drug; response to hyperoxia; response to lipopolysaccharide; transcription, DNA-dependent; viral process