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Western Blot (WB) (Western blot analysis of extracts from 293 cells, using XIAP (Ab-87) antibody.)

XIAP Antibody | anti-XIAP antibody

XIAP (Ab-87) Antibody

Gene Names
XIAP; API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
XIAP; XIAP (Ab-87) Antibody; API3; BIR4; BIRC4; Baculoviral IAP repeat-containing protein 4; HILP; IAP-like protein; IAP3; Inhibitor of apoptosis protein 3; X-linked IAP; X-linked inhibitor of apoptosis protein; anti-XIAP antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
XIAP (Ab-87) antibody detects endogenous levels of total XIAP protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
497
Applicable Applications for anti-XIAP antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human XIAP around the phosphorylation site of serine 87(K-V-SP-P-N).
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, using XIAP (Ab-87) antibody.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells, using XIAP (Ab-87) antibody.)
References
Andrew T. Ho, Mol. Cell. Biol., Aug 2007; 27: 5673-5685. Do-Sun Byun, Cancer Res., Nov 2003; 63: 7068-7075. Hamid Kashkar, J. Exp. Med., Jul 2003; 198: 341. Arnon P. Kater, Blood, Sep 2005; 106: 1742-1748.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
331
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
56,685 Da
NCBI Official Full Name
E3 ubiquitin-protein ligase XIAP
NCBI Official Synonym Full Names
X-linked inhibitor of apoptosis
NCBI Official Symbol
XIAP
NCBI Official Synonym Symbols
API3; ILP1; MIHA; XLP2; BIRC4; IAP-3; hIAP3; hIAP-3
NCBI Protein Information
E3 ubiquitin-protein ligase XIAP
UniProt Protein Name
E3 ubiquitin-protein ligase XIAP
Protein Family
UniProt Gene Name
XIAP
UniProt Synonym Gene Names
API3; BIRC4; IAP3; ILP; hILP; IAP-3; hIAP-3; hIAP3; X-linked IAP

NCBI Description

This gene encodes a protein that belongs to a family of apoptotic suppressor proteins. Members of this family share a conserved motif termed, baculovirus IAP repeat, which is necessary for their anti-apoptotic function. This protein functions through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2 and inhibits apoptosis induced by menadione, a potent inducer of free radicals, and interleukin 1-beta converting enzyme. This protein also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7. Mutations in this gene are the cause of X-linked lymphoproliferative syndrome. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 2 and 11.[provided by RefSeq, Feb 2011]

Uniprot Description

Multi-functional protein which regulates not only caspases and apoptosis, but also modulates inflammatory signaling and immunity, copper homeostasis, mitogenic kinase signaling, cell proliferation, as well as cell invasion and metastasis. Acts as a direct caspase inhibitor. Directly bind to the active site pocket of CASP3 and CASP7 and obstructs substrate entry. Inactivates CASP9 by keeping it in a monomeric, inactive state. Acts as an E3 ubiquitin-protein ligase regulating NF-kappa-B signaling and the target proteins for its E3 ubiquitin-protein ligase activity include: RIPK1, CASP3, CASP7, CASP8, CASP9, MAP3K2/MEKK2, DIABLO/SMAC, AIFM1, CCS and BIRC5/survivin. Ubiquitinion of CCS leads to enhancement of its chaperone activity toward its physiologic target, SOD1, rather than proteasomal degradation. Ubiquitinion of MAP3K2/MEKK2 and AIFM1 does not lead to proteasomal degradation. Plays a role in copper homeostasis by ubiquitinationg COMMD1 and promoting its proteasomal degradation. Can also function as E3 ubiquitin-protein ligase of the NEDD8 conjugation pathway, targeting effector caspases for neddylation and inactivation. Regulates the BMP signaling pathway and the SMAD and MAP3K7/TAK1 dependent pathways leading to NF-kappa-B and JNK activation. Acts as an important regulator of innate immune signaling via regulation of Nodlike receptors (NLRs). Protects cells from spontaneous formation of the ripoptosome, a large multi-protein complex that has the capability to kill cancer cells in a caspase-dependent and caspase-independent manner. Suppresses ripoptosome formation by ubiquitinating RIPK1 and CASP8. Acts as a positive regulator of Wnt signaling and ubiquitinates TLE1, TLE2, TLE3, TLE4 and AES. Ubiquitination of TLE3 results in inhibition of its interaction with TCF7L2/TCF4 thereby allowing efficient recruitment and binding of the transcriptional coactivator beta-catenin to TCF7L2/TCF4 that is required to initiate a Wnt-specific transcriptional program.

Research Articles on XIAP

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Product Notes

The XIAP xiap (Catalog #AAA8509526) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The XIAP (Ab-87) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's XIAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the XIAP xiap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XIAP, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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