WEE1 Antibody | anti-WEE1 antibody

WEE1 (Phospho-Ser642) Antibody

Cat. Num. AAA858225

• Gene Names: WEE1; WEE1A; WEE1hu
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: WEE1; WEE1 (Phospho-Ser642) Antibody; EC 2.7.10.2; kinase WEE1; Wee1-like protein kinase; WEE1A; WEE1hu; anti-WEE1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: WEE1 (Phospho-Ser642) antibody detects endogenous levels of WEE1 only when phosphorylated at serine 642.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 432

• Applicable Applications for anti-WEE1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human WEE1 around the phosphorylation site of serine 642 (S-V-SP-L-T).
• All Sites: Human: Ser642; Mouse: Ser642; Rat: Ser642
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells, treated with etoposide (25uM, 60mins), using WEE1 (Phospho-Ser642) antibody.)

Product Categories/Family for anti-WEE1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Watanabe N., EMBO J. 14:1878-1891(1995). Cichutek A., Cytogenet. Cell Genet. 93:277-283(2001). Igarashi M., Nature 353:80-83(1991).

NCBI and Uniprot Product Information

• NCBI GI #: 221307502
• NCBI GeneID: 7465
• NCBI Accession #: NP_001137448.1
• NCBI GenBank Nucleotide #: NM_001143976.1
• UniProt Accession #: P30291; B3KVE1; D3DQV0
• Molecular Weight: 49,072 Da
• NCBI Official Full Name: wee1-like protein kinase isoform 2
• NCBI Official Synonym Full Names: WEE1 G2 checkpoint kinase
• NCBI Official Symbol: WEE1
• NCBI Official Synonym Symbols: WEE1A; WEE1hu
• NCBI Protein Information: wee1-like protein kinase
• UniProt Protein Name: Wee1-like protein kinase
• Protein Family: Wee1-like protein kinase
• UniProt Gene Name: WEE1
• UniProt Synonym Gene Names: WEE1hu

NCBI Description

This gene encodes a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.