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Western Blot (WB) (Western blot analysis of extracts from HUVEC cells treated with PMA (125ng/ml, 30mins), and HUVEC cells treated with serum (20%, 30mins), using TNAP1 antibody.)

TNAP1 Antibody | anti-TNAP1 antibody

TNAP1 Antibody

Gene Names
TNFAIP1; B12; B61; EDP1; BTBD34; hBACURD2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
TNAP1; TNAP1 Antibody; B12; B61; EDP1; MGC2317; tumor necrosis factor; alpha-induced protein 1 (endothelial); anti-TNAP1 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
TNAP1 antibody detects endogenous levels of total TNAP1 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
316
Applicable Applications for anti-TNAP1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human TNAP1.
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HUVEC cells treated with PMA (125ng/ml, 30mins), and HUVEC cells treated with serum (20%, 30mins), using TNAP1 antibody.)

Western Blot (WB) (Western blot analysis of extracts from HUVEC cells treated with PMA (125ng/ml, 30mins), and HUVEC cells treated with serum (20%, 30mins), using TNAP1 antibody.)
References
Wolf F.W., J. Biol. Chem. 267:1317-1326(1992). The MGC Project Team, Genome Res. 14:2121-2127(2004). Nousiainen M., Proc. Natl. Acad. Sci. U.S.A. 103:5391-5396(2006).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,709 Da
NCBI Official Full Name
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
NCBI Official Synonym Full Names
TNF alpha induced protein 1
NCBI Official Symbol
TNFAIP1
NCBI Official Synonym Symbols
B12; B61; EDP1; BTBD34; hBACURD2
NCBI Protein Information
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
UniProt Protein Name
BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 2
UniProt Gene Name
TNFAIP1
UniProt Synonym Gene Names
BACURD2; EDP1; hBACURD2

NCBI Description

This gene was identified as a gene whose expression can be induced by the tumor necrosis factor alpha (TNF) in umbilical vein endothelial cells. Studies of a similar gene in mouse suggest that the expression of this gene is developmentally regulated in a tissue-specific manner. [provided by RefSeq, Jul 2008]

Uniprot Description

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in regulation of cytoskeleton structure. The BCR(TNFAIP1) E3 ubiquitin ligase complex mediates the ubiquitination of RHOA, leading to its degradation by the proteasome, thereby regulating the actin cytoskeleton and cell migration. Its interaction with RHOB may regulate apoptosis. May enhance the PCNA-dependent DNA polymerase delta activity.

Research Articles on TNAP1

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Product Notes

The TNAP1 tnfaip1 (Catalog #AAA851485) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The TNAP1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's TNAP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:1000 ELISA: 1:1000. Researchers should empirically determine the suitability of the TNAP1 tnfaip1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TNAP1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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