Rat Caspase 8 Associated Protein 2 (CASP8AP2) ELISA Kit | CASP8AP2 elisa kit

Rat Caspase 8 Associated Protein 2 (CASP8AP2) ELISA Kit

Cat. Num. AAA2000258

• Gene Names: CASP8AP2; CED-4; FLASH; RIP25
• Reactivity: Rat
• Synonyms: Caspase 8 Associated Protein 2 (CASP8AP2); Rat Caspase 8 Associated Protein 2 (CASP8AP2) ELISA Kit; CED-4; FLASH; RIP25; FLICE-Associated Huge Protein; CASP8AP2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Caspase 8 Associated Protein 2 (CASP8AP2).; No significant cross-reactivity or interference between Caspase 8 Associated Protein 2 (CASP8AP2) and analogues was observed.
• Sequence Length: 1982

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.136ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Caspase 8 Associated Protein 2 (CASP8AP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Caspase 8 Associated Protein 2 (CASP8AP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CASP8AP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 Associated Protein 2 (CASP8AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 Associated Protein 2 (CASP8AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 Associated Protein 2 (CASP8AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 8 Associated Protein 2 (CASP8AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CASP8AP2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 124376904
• NCBI GeneID: 9994
• UniProt Accession #: Q9UKL3; Q6PH76; Q7LCQ7; Q86YD9; Q9NUQ4; Q9NZV9; Q9P2N1; Q9Y563; A2RUB7; E1P553
• Molecular Weight: 222,658 Da
• NCBI Official Full Name: Caspase 8 associated protein 2
• NCBI Official Synonym Full Names: caspase 8 associated protein 2
• NCBI Official Symbol: CASP8AP2
• NCBI Official Synonym Symbols: CED-4; FLASH; RIP25
• NCBI Protein Information: CASP8-associated protein 2
• UniProt Protein Name: CASP8-associated protein 2
• Protein Family: CASP8-associated protein
• UniProt Gene Name: CASP8AP2
• UniProt Entry Name: C8AP2_HUMAN

NCBI Description

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008]

Uniprot Description

CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.

Protein type: Apoptosis; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: PML body; mitochondrion; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; SUMO polymer binding; death receptor binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity

Biological Process: caspase activation; induction of apoptosis via death domain receptors; regulation of transcription, DNA-dependent; transcription, DNA-dependent; cell cycle; signal transduction