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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using MAK (Phospho-Tyr159) antibody.)

MAK Antibody | anti-MAK antibody

MAK (Phospho-Tyr159) Antibody

Gene Names
MAK; RP62
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
MAK; MAK (Phospho-Tyr159) Antibody; EC 2.7.11.22; Male germ cell-associated kinase; Serine/threonine-protein kinase MAK; anti-MAK antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
MAK (Phospho-Tyr159) antibody detects endogenous levels of MAK only when phosphorylated at tyrosine 159.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
583
Applicable Applications for anti-MAK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:40000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human MAK around the phosphorylation site of tyrosine 159 (T-D-YP-V-S).
All Sites
Human: Tyr159
Mouse: Tyr159
Rat: Tyr159
Preparation and Storage
Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using MAK (Phospho-Tyr159) antibody.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, treated with PMA (125ng/ml, 30mins), using MAK (Phospho-Tyr159) antibody.)
References
Mungall A.J., Nature 425:805-811(2003). Matsushime H., Mol. Cell. Biol. 10:2261-2268(1990). Greenman C., Nature 446:153-158(2007).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,345 Da
NCBI Official Full Name
serine/threonine-protein kinase MAK isoform 2
NCBI Official Synonym Full Names
male germ cell associated kinase
NCBI Official Symbol
MAK
NCBI Official Synonym Symbols
RP62
NCBI Protein Information
serine/threonine-protein kinase MAK
UniProt Protein Name
Serine/threonine-protein kinase MAK
Protein Family
UniProt Gene Name
MAK

NCBI Description

The product of this gene is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. Mutations in this gene have been associated with ciliary defects resulting in retinitis pigmentosa 62. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]

Uniprot Description

Essential for the regulation of ciliary length and required for the long-term survival of photoreceptors (). Phosphorylates FZR1 in a cell cycle-dependent manner. Plays a role in the transcriptional coactivation of AR. Could play an important function in spermatogenesis. May play a role in chromosomal stability in prostate cancer cells.

Research Articles on MAK

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Product Notes

The MAK mak (Catalog #AAA854388) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MAK (Phospho-Tyr159) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:40000. Researchers should empirically determine the suitability of the MAK mak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAK, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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