Mouse Fibroblast Growth Factor-10 ELISA Kit | FGF-10 elisa kit

Mouse Fibroblast Growth Factor-10 ELISA Kit

Cat. Num. AAA43518

• Reactivity: Mouse
• Synonyms: Fibroblast Growth Factor-10; Mouse Fibroblast Growth Factor-10 ELISA Kit; FGF-10 elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes recombinant and natural Mouse FGF-10, no significant cross-reactivity or interference was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Detection Range: 15.6 pg/ml - 500 pg/ml.
• Sensitivity: 2.0 pg/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Mouse FGF-10 in the sample, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus Mouse FGF-10 concentration. The concentration of Mouse FGF-10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) are less than 15%.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF-10 elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4758360
• NCBI GeneID: 2255
• NCBI Accession #: NP_004456.1
• NCBI GenBank Nucleotide #: NM_004465.1
• UniProt Accession #: O15520; Q6FHT6; Q96P59
• Molecular Weight: 23,436 Da
• NCBI Official Full Name: fibroblast growth factor 10
• NCBI Official Synonym Full Names: fibroblast growth factor 10
• NCBI Official Symbol: FGF10
• NCBI Protein Information: fibroblast growth factor 10; FGF-10; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria
• UniProt Protein Name: Fibroblast growth factor 10
• UniProt Gene Name: FGF10
• UniProt Synonym Gene Names: FGF-10
• UniProt Entry Name: FGF10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein exhibits mitogenic activity for keratinizing epidermal cells, but essentially no activity for fibroblasts, which is similar to the biological activity of FGF7. Studies of the mouse homolog of suggested that this gene is required for embryonic epidermal morphogenesis including brain development, lung morphogenesis, and initiation of lim bud formation. This gene is also implicated to be a primary factor in the process of wound healing. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF10: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing. Interacts with FGFR1 and FGFR2. Interacts with FGFBP1. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted; Cell development/differentiation; Cytokine; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5p13-p12

Cellular Component: extracellular matrix; extracellular space; cell surface; extracellular region; plasma membrane; nucleus

Molecular Function: heparin binding; protein binding; growth factor activity; type 2 fibroblast growth factor receptor binding; fibroblast growth factor receptor binding; chemoattractant activity

Biological Process: nerve growth factor receptor signaling pathway; salivary gland development; activation of MAPK activity; urothelial cell proliferation; somatic stem cell maintenance; positive regulation of epithelial cell proliferation involved in wound healing; positive regulation of transcription, DNA-dependent; muscle cell fate commitment; response to lipopolysaccharide; regulation of saliva secretion; embryonic pattern specification; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; epithelial cell proliferation; positive regulation of fibroblast proliferation; positive chemotaxis; embryonic digestive tract morphogenesis; induction of an organ; mesonephros development; embryonic genitalia morphogenesis; spleen development; positive regulation of keratinocyte migration; positive regulation of DNA repair; fibroblast growth factor receptor signaling pathway; positive regulation of urothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; branching morphogenesis of a tube; positive regulation of transcription from RNA polymerase II promoter; smooth muscle cell differentiation; determination of left/right symmetry; metanephros development; positive regulation of epithelial cell proliferation; wound healing; radial glial cell differentiation; positive regulation of mitotic cell cycle; positive regulation of vascular endothelial growth factor receptor signaling pathway; response to estradiol stimulus; induction of positive chemotaxis; negative regulation of cell proliferation; establishment of mitotic spindle orientation; positive regulation of MAPKKK cascade; positive regulation of lymphocyte proliferation; tissue regeneration; pancreas development; male genitalia morphogenesis; thyroid gland development; lacrimal gland development; angiogenesis; otic vesicle formation; female genitalia morphogenesis; positive regulation of Notch signaling pathway; epidermal growth factor receptor signaling pathway; hair follicle morphogenesis; phosphoinositide-mediated signaling; thymus development; keratinocyte proliferation; regulation of activin receptor signaling pathway; embryonic camera-type eye development; odontogenesis of dentine-containing teeth; limb bud formation; pituitary gland development; positive regulation of ATPase activity; white fat cell differentiation; actin cytoskeleton reorganization; insulin receptor signaling pathway; innate immune response; blood vessel remodeling; positive regulation of Ras protein signal transduction; positive regulation of DNA replication; regulation of smoothened signaling pathway

Disease: Lacrimoauriculodentodigital Syndrome; Aplasia Of Lacrimal And Salivary Glands