GPR109 Antibody | anti-GPR109 antibody
GPR109 Antibody
Gene Names
HCAR3; HCA3; HM74; PUMAG; Puma-g; GPR109B
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
GPR109; GPR109 Antibody; Nicotinic acid receptor 2; G-protein coupled receptor 109B; G-protein coupled receptor HM74B; G-protein coupled receptor HM74; GPR109B; anti-GPR109 antibody
Reactivity
Human, Mouse, Rat
Specificity
GPR109 antibody detects endogenous levels of total GPR109 protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
387
Applicable Applications for anti-GPR109 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:10000
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from internal of human GPR109.
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
Western Blot (WB)
(Peptide-+ Western blot analysis of extracts from RAW264.7 cells, using GPR109 antibody. Immunofluorescence analysis of MCF-7 cells, using GPR109 antibody.)
Product Categories/Family for anti-GPR109 antibody
References
Nomura H., Int. Immunol. 5:1239-1249(1993). Ota T., Nat. Genet. 36:40-45(2004). Wise A., J. Biol. Chem. 278:9869-9874(2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44,478 Da
NCBI Official Full Name
hydroxycarboxylic acid receptor 3
NCBI Official Synonym Full Names
hydroxycarboxylic acid receptor 3
NCBI Official Symbol
HCAR3
NCBI Official Synonym Symbols
HCA3; HM74; PUMAG; Puma-g; GPR109B
NCBI Protein Information
hydroxycarboxylic acid receptor 3
UniProt Protein Name
Hydroxycarboxylic acid receptor 3
UniProt Gene Name
HCAR3
UniProt Synonym Gene Names
GPR109B; HCA3; HM74B; NIACR2
Uniprot Description
Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.
