Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eEF2 (Phospho-Thr56) antibody (#A0070).)

eEF2 Antibody | anti-eEF2 antibody

eEF2 (Phospho-Thr56) Antibody

Gene Names
EEF2; EF2; EF-2; EEF-2; SCA26
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
eEF2; eEF2 (Phospho-Thr56) Antibody; EEF2; EF-2; EF2; Elongation factor 2; anti-eEF2 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse, Rat
Specificity
eEF2 (Phospho-Thr56) antibody detects endogenous levels of eEF2 only when phosphorylated at threonine 56.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
858
Applicable Applications for anti-eEF2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1000
IHC: 1:50~100
IF: 1:100~1:500
ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human eEF2 around the phosphorylation site of threonine 56 (R-F-TP-D-T).
All Sites
Human: Thr56
Mouse: Thr56
Rat: Thr56
Preparation and Storage
Store at-20 degree C for 1 year

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eEF2 (Phospho-Thr56) antibody (#A0070).)

Immunohistochemistry (IHC) (P-peptide-+ Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eEF2 (Phospho-Thr56) antibody (#A0070).)

Western Blot (WB)

(Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30mins), using eEF2 (Phospho-Thr56) antibody (#A0070).)

Western Blot (WB) (Western blot analysis of extracts from NIH/3T3 cells treated with serum (10%, 30mins), using eEF2 (Phospho-Thr56) antibody (#A0070).)

Immunofluorescence (IF)

(P-peptide-+ Immunofluorescence analysis of HuvEc cells, using eEF2 (Phospho-Thr56) antibody (#A0070).)

Immunofluorescence (IF) (P-peptide-+ Immunofluorescence analysis of HuvEc cells, using eEF2 (Phospho-Thr56) antibody (#A0070).)
References
David M. Thomson, FASEB J, Mar 2006; 20: A819. Scott E. Gordon, FASEB J, Mar 2006; 20: A819. Xu Li, FEBS J., Aug 2005; 272: 4211-4220. Adam J. Rose, J. Physiol., Nov 2005; 569: 223-228.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
95,338 Da
NCBI Official Full Name
elongation factor 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 2
NCBI Official Symbol
EEF2
NCBI Official Synonym Symbols
EF2; EF-2; EEF-2; SCA26
NCBI Protein Information
elongation factor 2
UniProt Protein Name
Elongation factor 2
Protein Family
UniProt Gene Name
EEF2
UniProt Synonym Gene Names
EF2; EF-2

NCBI Description

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Research Articles on eEF2

Similar Products

Product Notes

The eEF2 eef2 (Catalog #AAA8509345) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The eEF2 (Phospho-Thr56) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's eEF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1000 IHC: 1:50~100 IF: 1:100~1:500 ELISA: 1:10000. Researchers should empirically determine the suitability of the eEF2 eef2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "eEF2, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.