E3K1 Antibody | anti-E3K1 antibody

E3K1 Antibody

Cat. Num. AAA856013

• Gene Names: SRPK1; SFRSK1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: E3K1; E3K1 Antibody; EC 2.7.11.1; Serine/arginine-rich protein-specific kinase 1; SR-protein-specific kinase 1; SFRS protein kinase 1; anti-E3K1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: SRPK1 antibody detects endogenous levels of total SRPK1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 655

• Applicable Applications for anti-E3K1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human SRPK1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data #2

(Western blot analysis of extracts from K562 cells and RAW264.7cells, using SRPK1 antibody.)

Product Categories/Family for anti-E3K1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Gui J.F., Nature 369:678-682(1994). Nikolakaki E., J. Biol. Chem. 276:40175-40182(2001). Mungall A.J., Nature 425:805-811(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 47419936
• NCBI GeneID: 6732
• NCBI Accession #: NP_003128.3
• NCBI GenBank Nucleotide #: NM_003137.4
• UniProt Accession #: Q96SB4; Q12890; Q5R364; Q5R365; Q8IY12; B4DS61
• Molecular Weight: 72,383 Da
• NCBI Official Full Name: SRSF protein kinase 1
• NCBI Official Synonym Full Names: SRSF protein kinase 1
• NCBI Official Symbol: SRPK1
• NCBI Official Synonym Symbols: SFRSK1
• NCBI Protein Information: SRSF protein kinase 1
• UniProt Protein Name: SRSF protein kinase 1
• UniProt Gene Name: SRPK1
• UniProt Synonym Gene Names: SR-protein-specific kinase 1

NCBI Description

This gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. Alternative splicing of this gene results in multiple transcript variants. Additional alternatively spliced transcript variants have been described for this gene, but their full length nature have not been determined.[provided by RefSeq, Jul 2010]

Uniprot Description

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Isoform 2 phosphorylates SFRS2, ZRSR2, LBR and PRM1. Isoform 2 phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Isoform 2 can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Isoform 1 and isoform 2 can induce splicing of exon 10 in MAPT/TAU. The ratio of isoform 1/isoform 2 plays a decisive role in determining cell fate in K-562 leukaemic cell line: isoform 2 favors proliferation where as isoform 1 favors differentiation.