E2A Antibody | anti-E2A antibody

E2A (Phospho-Thr355) Antibody

Cat. Num. AAA854292

• Gene Names: TCF3; E2A; E47; p75; AGM8; ITF1; VDIR; TCF-3; bHLHb21
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: E2A; E2A (Phospho-Thr355) Antibody; E12; Immunoglobulin enhancer binding factor E12/E47; Immunoglobulin transcription factor-1; ITF1; Kappa-E2-binding factor; TCF-3; TCF3; TFE2; transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47); Transcription factor E2-alpha; Transcription factor ITF-1; Transcription factor-3; anti-E2A antibody

• Reactivity: Human, Mouse, Rat
• Specificity: E2A (Phospho-Thr355) antibody detects endogenous levels of E2A only when phosphorylated at threonine 355.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 651

• Applicable Applications for anti-E2A antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human E2A around the phosphorylation site of threonine 355 (P-S-TP-P-V).
• All Sites: Human: Thr355; Mouse: Thr353; Rat: Thr351
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human brain tissue using E2A (Phospho-Thr355) antibody.)

Product Categories/Family for anti-E2A antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Kamps M.P., Cell 60:547-555(1990). Nourse J., Cell 60:535-545(1990). Grimwood J., Nature 428:529-535(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 209915594
• NCBI GeneID: 6929
• NCBI Accession #: NP_001129611.1
• NCBI GenBank Nucleotide #: NM_001136139.3
• UniProt Accession #: P15923; P15883; Q14208; Q14635; Q14636; Q2TB39; Q2TB40; Q9UPI9
• Molecular Weight: 68,510 Da
• NCBI Official Full Name: transcription factor E2-alpha isoform E47
• NCBI Official Synonym Full Names: transcription factor 3
• NCBI Official Symbol: TCF3
• NCBI Official Synonym Symbols: E2A; E47; p75; AGM8; ITF1; VDIR; TCF-3; bHLHb21
• NCBI Protein Information: transcription factor E2-alpha
• UniProt Protein Name: Transcription factor E2-alpha
• UniProt Gene Name: TCF3
• UniProt Synonym Gene Names: BHLHB21; E2A; ITF1; bHLHb21; TCF-3

NCBI Description

This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. E proteins play a critical role in lymphopoiesis, and the encoded protein is required for B and T lymphocyte development. Deletion of this gene or diminished activity of the encoded protein may play a role in lymphoid malignancies. This gene is also involved in several chromosomal translocations that are associated with lymphoid malignancies including pre-B-cell acute lymphoblastic leukemia (t(1;19), with PBX1), childhood leukemia (t(19;19), with TFPT) and acute leukemia (t(12;19), with ZNF384). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Sep 2011]

Uniprot Description

Transcriptional regulator. Involved in the initiation of neuronal differentiation. Heterodimers between TCF3 and tissue-specific basic helix-loop-helix (bHLH) proteins play major roles in determining tissue-specific cell fate during embryogenesis, like muscle or early B-cell differentiation. Dimers bind DNA on E-box motifs: 5'-CANNTG-3'. Binds to the kappa-E2 site in the kappa immunoglobulin gene enhancer. Binds to IEB1 and IEB2, which are short DNA sequences in the insulin gene transcription control region.