Testing Data
(Peptide-+)
CPI17alpha Antibody | anti-CPI17alpha antibody
CPI17alpha (Ab-38) Antibody
Gene Names
PPP1R14A; CPI17; CPI-17; PPP1INL
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
CPI17alpha; CPI17alpha (Ab-38) Antibody; 17-kDa PKC-potentiated inhibitory protein of PP1; CPI; CPI-17; CPI17; PKC-POTENTIATED PP1 inhibitory protein; PKC-potentiated PP1 inhibitory protein; PP14A; Protein phosphatase 1; regulatory (Inhibitor) subunit 14A; anti-CPI17alpha antibody
Reactivity
Human, Mouse, Rat
Specificity
CPI17alpha (Ab-38) antibody detects endogenous levels of total CPI17alpha protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
120
Applicable Applications for anti-CPI17alpha antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
IHC: 1:50~1:100
IF: 1:100~1:500
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human CPI17alpha around the phosphorylation site of threonine 38 (R-V-TP-V-K).
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
(Peptide-+)
Testing Data
(Peptide-+)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human heart tissue using CPI17alpha (Ab-38) antibody. Western blot analysis of extracts from HT-29 cells, using CPI17alpha (Ab-38) antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis of paraffin-embedded human heart tissue using CPI17alpha (Ab-38) antibody. Western blot analysis of extracts from HT-29 cells, using CPI17alpha (Ab-38) antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of HeLa cells, using CPI17alpha (Ab-38) antibody.)
Immunofluorescence (IF)
(Peptide-+ Immunofluorescence analysis of HeLa cells, using CPI17alpha (Ab-38) antibody.)
Product Categories/Family for anti-CPI17alpha antibody
References
Yamawaki K., Biochem. Biophys. Res. Commun. 285:1040-1045(2001). Cui W.C., Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases. Liu Q.-R., Biochem. J. 377:171-181(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,480 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 14A isoform 2
NCBI Official Synonym Full Names
protein phosphatase 1 regulatory inhibitor subunit 14A
NCBI Official Symbol
PPP1R14A
NCBI Official Synonym Symbols
CPI17; CPI-17; PPP1INL
NCBI Protein Information
protein phosphatase 1 regulatory subunit 14A
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 14A
UniProt Gene Name
PPP1R14A
UniProt Synonym Gene Names
CPI17; PPP1INL; CPI-17
NCBI Description
The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]
Uniprot Description
Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.
Research Articles on CPI17alpha
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Product Notes
The CPI17alpha ppp1r14a (Catalog #AAA859217) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The CPI17alpha (Ab-38) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CPI17alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the CPI17alpha ppp1r14a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CPI17alpha, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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