Pim-3 Oncogene Recombinant Protein | PIM3 recombinant protein

Recombinant Pim-3 Oncogene (PIM3)

Cat. Num. AAA2033173

• Gene Names: PIM3; pim-3
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Pim-3 Oncogene; Recombinant Pim-3 Oncogene (PIM3); Serine/threonine-protein kinase pim-3; PIM3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MLLSKFGSLA HLCGPGGVDH LPVKILQPAK ADKESFEKAY QVGAVLGSGG FGTVYAGSRI ADGLPVAVKH VVKERVTEWG SLGGATVPLE VVLLRKVGAA GGARGVIRLL DWFERPDGFL LVLERPEPAQ DLFDFITERG ALDEPLARRF FAQVLAAVRH CHSCGVVHRD IKDENLLVDL RSGELKLIDF GSGALLKDTV YTDFDGTRVY SPPEWIRYHR YHGRSATVWS LGVLLYDMVC GDIPFEQDEE ILRGRLLFRR RVSPECQQLI RWCLSLRPSE RPSLDQIAAH PWMLGADGGVPESCDLRLCT LDPDDVASTT SSSESL
• Sequence Length: 326

• Applicable Applications for PIM3 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 39.6kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met1~Leu326
• Organism Species: Human
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 146327819
• NCBI GeneID: 415116
• Molecular Weight: 35,891 Da
• NCBI Official Full Name: Pim-3 oncogene
• NCBI Official Synonym Full Names: Pim-3 proto-oncogene, serine/threonine kinase
• NCBI Official Symbol: PIM3
• NCBI Official Synonym Symbols: pim-3
• NCBI Protein Information: serine/threonine-protein kinase pim-3
• UniProt Protein Name: Serine/threonine-protein kinase pim-3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PIM3
• UniProt Entry Name: PIM3_HUMAN

NCBI Description

The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012]

Uniprot Description

Pim3: a Ca(2 )/calmodulin-dependent protein kinase of the PIM family. Highly expressed in hematopoietic tissues, in leukemia and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells.

Protein type: Kinase, protein; Protein kinase, CAMK; Oncoprotein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); CAMK group; PIM family

Chromosomal Location of Human Ortholog: 22q13

Cellular Component: cytoplasm

Molecular Function: ATP binding; protein binding; protein serine/threonine kinase activity

Biological Process: apoptosis; cell cycle; negative regulation of apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of mitotic cell cycle