Western Blot (WB)
(Western blot analysis on HeLa cell lysate using Phospho-CDC25A (Ser75) Antibody)
CDC25A Antibody | anti-CDC25A antibody
Phospho-CDC25A (Ser75) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified
Synonyms
CDC25A; Phospho-CDC25A (Ser75) Antibody; CDC25M3; Dual specificity phosphatase Cdc25A; EC 3.1.3.48; M-phase inducer phosphatase 1; MPIP1; anti-CDC25A antibody
Reactivity
Human, Mouse, Rat
Specificity
Phospho-CDC25A(Ser75) Antibody detects endogenous levels of CDC25A only when phosphorylated at Sersine 75
Purity/Purification
Immunogen affinity purified
Form/Format
In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
524
Applicable Applications for anti-CDC25A antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide derived from human CDC25A around the phosphorylation site of Sersine 75
Preparation and Storage
Store at-20 degree C for 1 year
Western Blot (WB)
(Western blot analysis on HeLa cell lysate using Phospho-CDC25A (Ser75) Antibody)
Western Blot (WB)
(Western blot analysis on HeLa cell lysate using Phospho-CDC25A (Ser75) Antibody)
Related Product Information for anti-CDC25A antibody
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59kDa
NCBI Official Full Name
M-phase inducer phosphatase 1 isoform a
NCBI Official Synonym Full Names
cell division cycle 25A
NCBI Official Symbol
CDC25A
NCBI Official Synonym Symbols
CDC25A2
NCBI Protein Information
M-phase inducer phosphatase 1
UniProt Protein Name
M-phase inducer phosphatase 1
Protein Family
UniProt Gene Name
CDC25A
NCBI Description
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro.
Research Articles on CDC25A
Similar Products
Product Notes
The CDC25A cdc25a (Catalog #AAA8510563) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Phospho-CDC25A (Ser75) Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CDC25A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the CDC25A cdc25a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CDC25A, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
