CARM1 Antibody | anti-CARM1 antibody

CARM1 (Phospho-Ser228) antibody

Cat. Num. AAA856738

• Gene Names: CARM1; PRMT4
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: CARM1; CARM1 (Phospho-Ser228) antibody; anti-CARM1 antibody

• Reactivity: Human, Mouse, Rat
• Specificity: CARM1 (Phospho-Ser228) antibody detects endogenous levels of CARM1 only when phosphorylated at serine 228.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 608

• Applicable Applications for anti-CARM1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human CARM1 around the phosphorylation site of serine 228 (V-K-SP-N-N).
• Preparation and Storage: Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 (Ab-228) antibody and CARM1 (Phospho-Ser228) antibody.)

Product Categories/Family for anti-CARM1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Selma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103(36): 13351-13356. Marcela Covic, et al. (2005) EMBO J; 24(1): 85-96. Eleni Zika et al. (2005) Proc Natl Acad Sci U S A.; 102(45): 16321-16326 Neelu Yadav et al. (2003) Proc Natl Acad Sci U S A; 100(11): 6464-6468.

NCBI and Uniprot Product Information

• NCBI GI #: 40288288
• NCBI GeneID: 10498
• NCBI Accession #: NP_954592.1
• NCBI GenBank Nucleotide #: NM_199141.1
• UniProt Accession #: Q86X55; A6NN38
• Molecular Weight: 41,885 Da
• NCBI Official Full Name: histone-arginine methyltransferase CARM1
• NCBI Official Synonym Full Names: coactivator associated arginine methyltransferase 1
• NCBI Official Symbol: CARM1
• NCBI Official Synonym Symbols: PRMT4
• NCBI Protein Information: histone-arginine methyltransferase CARM1
• UniProt Protein Name: Histone-arginine methyltransferase CARM1
• Protein Family: Histone-arginine methyltransferase
• UniProt Gene Name: CARM1
• UniProt Synonym Gene Names: PRMT4

NCBI Description

This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts specifically on histones and other chromatin-associated proteins and is involved in regulation of gene expression. The enzyme may act in association with other proteins or within multi-protein complexes and may play a role in cell type-specific functions and cell lineage specification. A related pseudogene is located on chromosome 9. [provided by RefSeq, Aug 2013]

Uniprot Description

Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.