Profilin Phospho-Regulation Antibody Sampler Kit
Profilin Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
Profilin Phospho-Regulation; ProfilinK; Profilin Phospho-Regulation antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline (PBS), 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for Profilin Phospho-Regulation antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C. Stable for 1year.
Related Product Information for Profilin Phospho-Regulation antibody sampler kit
Kit Summary: The Profilin phospho-regulation antibody sampler kit can be used to detect phosphorylation of Tyr-129 and Ser-138relative to total profilin expression levels. The kit includes rabbit polyclonal phospho-specific antibodies to Tyr-129 andSer-138, as well as rabbit polyclonal antibodies that detect different epitopes in the C-terminal region of Profilin. The kitalso includes an anti-Rabbit Light Chain specific:HRP secondary reagent for detection of rabbit polyclonal antibody in western blot, ELISA, or immunocytochemistry.
Background: Profilins are small actin-binding proteins that have functions in cell motility, cytokinesis, gene transcription, endocytosisand neuronal plasticity. Four profilin isoforms have been identified in mammals. Profilin-1 (PFN1) and profilin-2a (PFN2a)isoforms are highly conserved in structure, but PFN1 is ubiquitously expressed while PFN2a is preferentially enriched inbrain. In addition, there are two testis-specific profilins, PFN3 and PFN4, that significantly differ in primary sequence andfunction compared to PFN1 and PFN2a. Profilin is phosphorylated at both tyrosine and serine residues in vivo. Tyr-129 isphosphorylated in response to VEGF-A stimulation, and this promotes profilin actin binding and polymerization. Tyr-129phosphorylation may be important for angiogenesis induced by injuries. Ser-138 is phosphorylated by ROCK anddephosphorylated by PP1. This serine phosphorylation inhibits G-actin binding, as well as decreases profilin'saggregation suppressor activity by inhibiting binding to huntingtin. Thus, Tyr-129 phosphorylation may activate while Ser-138 phosphorylation may inhibit profilin activity.
Background: Profilins are small actin-binding proteins that have functions in cell motility, cytokinesis, gene transcription, endocytosisand neuronal plasticity. Four profilin isoforms have been identified in mammals. Profilin-1 (PFN1) and profilin-2a (PFN2a)isoforms are highly conserved in structure, but PFN1 is ubiquitously expressed while PFN2a is preferentially enriched inbrain. In addition, there are two testis-specific profilins, PFN3 and PFN4, that significantly differ in primary sequence andfunction compared to PFN1 and PFN2a. Profilin is phosphorylated at both tyrosine and serine residues in vivo. Tyr-129 isphosphorylated in response to VEGF-A stimulation, and this promotes profilin actin binding and polymerization. Tyr-129phosphorylation may be important for angiogenesis induced by injuries. Ser-138 is phosphorylated by ROCK anddephosphorylated by PP1. This serine phosphorylation inhibits G-actin binding, as well as decreases profilin'saggregation suppressor activity by inhibiting binding to huntingtin. Thus, Tyr-129 phosphorylation may activate while Ser-138 phosphorylation may inhibit profilin activity.
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Product Notes
The Human, Rat, Mouse Profilin Phospho-Regulation (Catalog #AAA474497) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474497 Antibody Sampler Kit recognizes Human, Rat, Mouse Profilin Phospho-Regulation. AAA Biotech's Profilin Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the Profilin Phospho-Regulation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Profilin Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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