c-Src Phospho-Regulation Antibody Sampler Kit | c-Src antibody sampler kit
c-Src Phospho-Regulation
Reactivity
Human, Rat, Mouse
Applications
Western Blot, ELISA
Synonyms
c-Src Phospho-Regulation; c-Src antibody sampler kit
Reactivity
Human, Rat, Mouse
Form/Format
Mouse monoclonal and rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1mg/ml BSA, and 0.05% sodium azide. The secondary reagents are supplied in the same buffer without azide.
Applicable Applications for c-Src antibody sampler kit
Western Blot (WB), ELISA (EIA)
Preparation and Storage
Store all at -20 degree C.Stable for 1 year.
Related Product Information for c-Src antibody sampler kit
Kit Summary: The c-Src phospho-regulation antibody sampler kit can be used to detect c-Src phosphorylation on Tyr-215 and Tyr-530. The kit also includes a monoclonal antibody to monitor total c-Src expression levels and secondary reagents for detectionof these antibodies.
Background: c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed ofnine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transducesignals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, andadhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. Theactivity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation.Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 down regulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
Background: c-Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. The Src family is composed ofnine members in vertebrates, including c-Src, Yes, Fgr, Yrk, Fyn, Lyn, Hck, Lck, and Blk. Src-family kinases transducesignals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, andadhesion. Src-family kinases contain an N-terminal cell membrane anchor followed by SH3 and SH2 domains. Theactivity of c-Src is regulated by tyrosine phosphorylation at multiple sites. Tyrosine 418 is autophosphorylated following c-Src activation. Tyrosine 215 in the SH2 domain of c-Src is phosphorylated following growth factor receptor activation.Both Tyr-215 and Tyr-418 phosphorylation increases tyrosine kinase activity, while phosphorylation of Tyr-530 down regulates c-Src kinase activity. Thus, tyrosine phosphorylation of c-Src is critical for regulating its kinase activity.
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Product Notes
The Human, Rat, Mouse c-Src (Catalog #AAA474569) is an Antibody Sampler Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA474569 Antibody Sampler Kit recognizes Human, Rat, Mouse c-Src. AAA Biotech's c-Src Phospho-Regulation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the c-Src for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-Src Phospho-Regulation, Antibody Sampler Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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