Human, Mouse TIE2 ELISA Kit | TIE2 elisa kit
Phospho-TIE2 (Tyr1108) Colorimetric Cell-Based ELISA Kit
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]
Uniprot Description
TIE2: a transmembrane tyrosine-protein kinase receptor for ANGPT1, ANGPT2 and ANGPT4. Regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Expressed almost exclusively in endothelial cells. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of FAK and of the downstream kinases ERK2 and ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. ANGPT2 has lower affinity for TEK, promoting TEK autophosphorylation in the absence of ANGPT1, but inhibiting ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. Three isoforms of the human protein are produced by alternative splicing.
Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group; Tie family
Chromosomal Location of Human Ortholog: 9p21.2
Cellular Component: actin filament; apical plasma membrane; basal plasma membrane; basolateral plasma membrane; cell surface; cytoplasm; extracellular region; focal adhesion; integral component of plasma membrane; intercellular junction; membrane raft; microtubule organizing center; microvillus; plasma membrane; stress fiber
Molecular Function: ATP binding; growth factor binding; protein binding; protein kinase activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor activity; transmembrane receptor protein tyrosine kinase activity
Biological Process: angiogenesis; cell-cell signaling; endochondral ossification; endothelial cell proliferation; glomerulus vasculature development; heart development; leukocyte migration; MAPK cascade; negative regulation of angiogenesis; negative regulation of apoptosis; negative regulation of endothelial cell apoptotic process; negative regulation of inflammatory response; peptidyl-tyrosine phosphorylation; positive regulation of actin cytoskeleton reorganization; positive regulation of angiogenesis; positive regulation of endothelial cell migration; positive regulation of endothelial cell proliferation; positive regulation of ERK1 and ERK2 cascade; positive regulation of focal adhesion assembly; positive regulation of intracellular signal transduction; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; protein autophosphorylation; protein oligomerization; regulation of endothelial cell apoptotic process; regulation of establishment or maintenance of cell polarity; regulation of vascular permeability; response to cAMP; response to estrogen; response to hypoxia; response to peptide hormone; signal transduction; sprouting angiogenesis; Tie signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway
Disease: Glaucoma 3, Primary Congenital, E; Glaucoma 3, Primary Infantile, B; Venous Malformations, Multiple Cutaneous And Mucosal
Research Articles on TIE2
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Product Notes
The Human, Mouse TIE2 tek (Catalog #AAA9501165) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9501165 ELISA Kit recognizes Human, Mouse TIE2. It is sometimes possible for the material contained within the vial of "TIE2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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