Human, Mouse Elk1 ELISA Kit | ELK1 elisa kit

Elk1 (Phospho-Ser389) TFact DNA-Binding ELISA Kit

Cat. Num. AAA9502155

• Reactivity: Human, Mouse
• Synonyms: Elk1; Elk1 (Phospho-Ser389) TFact DNA-Binding ELISA Kit; ELK1; ETS-domain protein ELK-1; ETS-domain protein Elk-1; ELK1 elisa kit

• Reactivity: Human, Mouse
• Specificity: The TFact Elk1 (Phospho-Ser389) DNA-Binding ELISA Kit detects endogenous levels of Elk1 only when phosphorylated at Ser389.
• Form/Format: 12 x 8-Well Microstrips
• Sequence Length: 428

• Samples: Cell lysate
• Assay Type: DNA-Binding ELISA
• Sensitivity: 10-30 ug of total protein/well
• Detection Method: Colorimetric 450 nm
• Subtype: Phospho
• Preparation and Storage: Store at 4 degree C for 6 months.

Testing Data

(The TFact Elk1 (Phospho-Ser389) DNA-Binding ELISA detects active Elk1 (Phospho-Ser389) in Jurkat Nuclear Extract. The Jurkat cells were grown 3 days in RPMI 1640 with 10% FBS and harvested for nuclear extract. The DNA-transcription factor complex is treated with stablization buffer. )

NCBI and Uniprot Product Information

• NCBI GI #: 166362732
• NCBI GeneID: 2002
• NCBI Accession #: NP_001107595.1
• NCBI GenBank Nucleotide #: NM_001114123.2
• UniProt Accession #: P19419; O75606; O95058; Q969X8; Q9UJM4; B2R7H4
• Molecular Weight: 11,217 Da
• NCBI Official Full Name: ETS domain-containing protein Elk-1 isoform a
• NCBI Official Synonym Full Names: ELK1, ETS transcription factor
• NCBI Official Symbol: ELK1
• NCBI Protein Information: ETS domain-containing protein Elk-1
• UniProt Protein Name: ETS domain-containing protein Elk-1
• Protein Family: ETS domain-containing protein
• UniProt Gene Name: ELK1

NCBI Description

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

Uniprot Description

ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: axon terminus; cell soma; dendrite; mitochondrion; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding transcription factor activity; protein binding

Biological Process: cell differentiation; cellular response to gamma radiation; cellular response to testosterone stimulus; positive regulation of neuron death; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; response to fibroblast growth factor; response to light stimulus; transcription from RNA polymerase II promoter