anti-Human, Mouse UPF1 Antibody | anti-UPF1 antibody

UPF1 Antibody

Cat. Num. AAA8509927

• Gene Names: UPF1; HUPF1; NORF1; RENT1; smg-2; pNORF1
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: UPF1; UPF1 Antibody; EC 3.6.1.-; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1; anti-UPF1 antibody

• Reactivity: Human, Mouse
• Specificity: UPF1 antibody detects endogenous levels of total UPF1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1129

• Applicable Applications for anti-UPF1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Immunogen: The antiserum was produced against synthesized peptide derived from internal of human UPF1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(Peptide-+)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using UPF1 antibody. Western blot analysis of extracts from K562 cells and NIH/3T3 cells, using UPF1 antibody. For Research Use Only)

References

Perlick H.A., Proc. Natl. Acad. Sci. U.S.A. 93:10928-10932(1996). Applequist S.E., Nucleic Acids Res. 25:814-821(1997). Nagase T., DNA Res. 3:321-329(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 662033888
• NCBI GeneID: 5976
• NCBI Accession #: NP_001284478.1
• NCBI GenBank Nucleotide #: NM_001297549.1
• UniProt Accession #: Q92900; O00239; O43343; Q86Z25; Q92842
• Molecular Weight: 123,036 Da
• NCBI Official Full Name: regulator of nonsense transcripts 1 isoform 1
• NCBI Official Synonym Full Names: UPF1, RNA helicase and ATPase
• NCBI Official Symbol: UPF1
• NCBI Official Synonym Symbols: HUPF1; NORF1; RENT1; smg-2; pNORF1
• NCBI Protein Information: regulator of nonsense transcripts 1
• UniProt Protein Name: Regulator of nonsense transcripts 1
• Protein Family: ATP-dependent helicase
• UniProt Gene Name: UPF1
• UniProt Synonym Gene Names: KIAA0221; RENT1; NORF1; hUpf1

NCBI Description

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]

Uniprot Description

RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation and allowing the recycling of NMD factors. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD. Essential for embryonic viability.