anti-Human, Mouse RapGEF1 Antibody | anti-RapGEF1 antibody

RapGEF1 (Phospho-Tyr504) Antibody

Cat. Num. AAA858573

• Gene Names: RAPGEF1; C3G; GRF2
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: RapGEF1; RapGEF1 (Phospho-Tyr504) Antibody; C3G; CRK SH3-binding GNRP; GRF2; RPGF1; Rap guanine nucleotide exchange factor 1; guanine nucleotide-releasing factor 2; anti-RapGEF1 antibody

• Reactivity: Human, Mouse
• Specificity: RapGEF1 (Phospho-Tyr504) antibody detects endogenous levels of RapGEF1 only when phosphorylated at tyrosine 504.
• Purity/Purification: Affinity-purified fromrabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1094

• Applicable Applications for anti-RapGEF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:1000; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human RapGEF1 around the phosphorylation site of tyrosine 504 (V-P-YP-A-P).
• All Sites: Human: Tyr504; Mouse: Tyr514
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, treated with Na3VO4 (0.3nM, 40mins), using RapGEF1 (Phospho-Tyr504) antibody.)

Product Categories/Family for anti-RapGEF1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

Tanaka S., Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994). Knudsen B., J. Biol. Chem. 269:32781-32787(1994). Matsuda M., J. Biol. Chem. 271:14468-14472(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 747019253
• NCBI GeneID: 2889
• NCBI Accession #: NP_001291204.1
• NCBI GenBank Nucleotide #: NM_001304275.1
• UniProt Accession #: Q13905; Q5JUE4; Q68DL3; Q8IV73
• Molecular Weight: 122,287 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 1 isoform c
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor 1
• NCBI Official Symbol: RAPGEF1
• NCBI Official Synonym Symbols: C3G; GRF2
• NCBI Protein Information: rap guanine nucleotide exchange factor 1
• UniProt Protein Name: Rap guanine nucleotide exchange factor 1
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF1
• UniProt Synonym Gene Names: GRF2

NCBI Description

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.