anti-Human RapGEF1 Antibody | anti-RapGEF1 antibody

RapGEF1 Antibody

Cat. Num. AAA8509780

• Gene Names: RAPGEF1; C3G; GRF2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: RapGEF1; RapGEF1 Antibody; C3G; CRK SH3-binding GNRP; GRF2; RPGF1; Rap guanine nucleotide exchange factor 1; guanine nucleotide-releasing factor 2; anti-RapGEF1 antibody

• Reactivity: Human
• Specificity: RapGEF1 antibody detects endogenous levels of total RapGEF1 protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 1094

• Applicable Applications for anti-RapGEF1 antibody: Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1000
• Immunogen: The antiserum was produced against synthesized peptide derived from Internal of human RapGEF1.
• Preparation and Storage: Store at-20 degree C for 1 year.

Immunohistochemistry (IHC)

(Peptide-+ Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using RapGEF1 antibody.)

References

Tanaka S., Proc. Natl. Acad. Sci. U.S.A. 91:3443-3447(1994). Knudsen B., J. Biol. Chem. 269:32781-32787(1994). Humphray S.J., Nature 429:369-374(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 747019253
• NCBI GeneID: 2889
• NCBI Accession #: NP_001291204.1
• NCBI GenBank Nucleotide #: NM_001304275.1
• UniProt Accession #: Q13905; Q5JUE4; Q68DL3; Q8IV73
• Molecular Weight: 122,287 Da
• NCBI Official Full Name: rap guanine nucleotide exchange factor 1 isoform c
• NCBI Official Synonym Full Names: Rap guanine nucleotide exchange factor 1
• NCBI Official Symbol: RAPGEF1
• NCBI Official Synonym Symbols: C3G; GRF2
• NCBI Protein Information: rap guanine nucleotide exchange factor 1
• UniProt Protein Name: Rap guanine nucleotide exchange factor 1
• Protein Family: Rap guanine nucleotide exchange factor
• UniProt Gene Name: RAPGEF1
• UniProt Synonym Gene Names: GRF2

NCBI Description

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

Guanine nucleotide-releasing protein that binds to SH3 domain of CRK and GRB2/ASH. Transduces signals from CRK to activate RAS. Plays a role in the establishment of basal endothelial barrier function. Plays a role in nerve growth factor (NGF)-induced sustained activation of Rap1 and neurite outgrowth.