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Testing Data

anti-Human, Mouse NEK9 Antibody | anti-NEK9 antibody

NEK9 (Phospho-Thr210) Antibody

Gene Names
NEK9; NC; APUG; NERCC; LCCS10; NERCC1
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
NEK9; NEK9 (Phospho-Thr210) Antibody; EC 2.7.11.1; KIAA1995; NERCC; NIMA-related kinase 8; Nek8; Nercc1 kinase; NimA-related protein kinase 9; Serine/threonine-protein kinase Nek9; anti-NEK9 antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
NEK9 (Phospho-Thr210) antibody detects endogenous levels of NEK9 only when phosphorylated at threonine 210.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
991
Applicable Applications for anti-NEK9 antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human NEK9 around the phosphorylation site of threonine 210(A-E-TP-L-V).
All Sites
Human: Thr210
Mouse: Thr210
Preparation and Storage
Store at-20 degree C for 1 year.

Testing Data

Testing Data

Western Blot (WB)

(P-peptide-+ Western blot analysis of extracts from HepG2 cells, using NEK9 (Phospho-Thr210) antibody. Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) antibody.)

Western Blot (WB) (P-peptide-+ Western blot analysis of extracts from HepG2 cells, using NEK9 (Phospho-Thr210) antibody. Immunofluorescence analysis of HeLa cells, using NEK9 (Phospho-Thr210) antibody.)
References
Holland P.M., J. Biol. Chem. 277:16229-16240(2002). Roig J., Genes Dev. 16:1640-1658(2002). Ohara O., DNA Res. 9:47-57(2002).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
107,168 Da
NCBI Official Full Name
serine/threonine-protein kinase Nek9 isoform 1
NCBI Official Synonym Full Names
NIMA related kinase 9
NCBI Official Symbol
NEK9
NCBI Official Synonym Symbols
NC; APUG; NERCC; LCCS10; NERCC1
NCBI Protein Information
serine/threonine-protein kinase Nek9
UniProt Protein Name
Serine/threonine-protein kinase Nek9
UniProt Gene Name
NEK9
UniProt Synonym Gene Names
KIAA1995; NEK8; NERCC; NimA-related protein kinase 9; Nek8

NCBI Description

This gene encodes a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. The encoded protein is activated in mitosis and, in turn, activates other family members during mitosis. This protein also mediates cellular processes that are essential for interphase progression. [provided by RefSeq, Jul 2016]

Uniprot Description

Pleiotropic regulator of mitotic progression, participating in the control of spindle dynamics and chromosome separation. Phosphorylates different histones, myelin basic protein, beta-casein, and BICD2. Phosphorylates histone H3 on serine and threonine residues and beta-casein on serine residues. Important for G1/S transition and S phase progression. Phosphorylates NEK6 and NEK7 and stimulates their activity by releasing the autoinhibitory functions of Tyr-108 and Tyr-97 respectively.

Research Articles on NEK9

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Product Notes

The NEK9 nek9 (Catalog #AAA859643) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The NEK9 (Phospho-Thr210) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NEK9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:20000. Researchers should empirically determine the suitability of the NEK9 nek9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEK9, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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