Immunohistochemistry (IHC)
(MBS9603393 at 1/100 staining rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
anti-Human, Mouse MYH4-Specific Antibody | anti-MYH4 antibody
MYH4-Specific Antibody
Gene Names
MYH4; MYH2B; MyHC-2B; MyHC-IIb
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Synonyms
MYH4-Specific; MYH4-Specific Antibody; fetal; MYH2B; Myh4; MYH4_HUMAN; MyHC-2b; MyHC-IIb; Myosin heavy chain 2b; Myosin heavy chain 4; Myosin heavy chain 4 skeletal muscle; Myosin heavy chain; Myosin heavy chain IIb; Myosin heavy chain skeletal muscle fetal; Myosin-4; skeletal muscle; anti-MYH4 antibody
Reactivity
Human, Mouse
Specificity
MYH4 Antibody detects endogenous levels of total MYH4.
Form/Format
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-MYH4 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (Peptide)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20000-1:40000
IHC: 1:50-1:200
ELISA(peptide): 1:20000-1:40000
Immunogen
A synthesized peptide derived from human MYH4, corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603393 at 1/100 staining rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(MBS9603393 at 1/100 staining rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse skeletal muscle tissue, using MYH4-Specific antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse skeletal muscle tissue, using MYH4-Specific antibody.)
Western Blot (WB)
(Western blot analysis of extracts from mouse lung, using MYH4-Specific antibody. Lane 1 was treated with the blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from mouse lung, using MYH4-Specific antibody. Lane 1 was treated with the blocking peptide.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
230kd
NCBI Official Full Name
myosin-4
NCBI Official Synonym Full Names
myosin heavy chain 4
NCBI Official Symbol
MYH4
NCBI Official Synonym Symbols
MYH2B; MyHC-2B; MyHC-IIb
NCBI Protein Information
myosin-4
UniProt Protein Name
Myosin-4
Protein Family
UniProt Gene Name
MYH4
UniProt Synonym Gene Names
MyHC-2b; MyHC-IIb
Uniprot Description
Muscle contraction.CautionRepresents a conventional myosin. This protein should not be confused with the unconventional myosin-4 (MYO4).
Research Articles on MYH4
Similar Products
Product Notes
The MYH4 myh4 (Catalog #AAA9603393) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The MYH4-Specific Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MYH4-Specific can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (Peptide). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20000-1:40000. Researchers should empirically determine the suitability of the MYH4 myh4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYH4-Specific, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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