Western Blot (WB)
(Western blot analysis of HSD17B8 using A549 whole cell lysates)
Rabbit anti-Human HSD17B8 Polyclonal Antibody | anti-HSD17B8 antibody
HSD17B8 Antibody
Gene Names
HSD17B8; KE6; FABG; HKE6; FABGL; RING2; H2-KE6; SDR30C1; D6S2245E; dJ1033B10.9
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
HSD17B8; Polyclonal Antibody; HSD17B8 Antibody; 17 beta HSD 8; 17 beta hydroxysteroid dehydrogenase 8; 17-beta-HSD 8; 17-beta-hydroxysteroid dehydrogenase 8; 17-beta-hydroxysteroid dehydrogenase VIII; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-oxoacyl-acyl-carrier protein reductase; E. coli; homolog of; Beta ketoacyl [acyl carrier protein] reductase like; D6S2245E; DHB8_HUMAN; dJ1033B10.9; Estradiol 17 beta dehydrogenase 8; Estradiol 17-beta-dehydrogenase 8; Estrogen 17 oxidoreductase; FABG; FabG; FabG-like; FABGL; H2 KE6; H2-KE6; HKE6; Hydroxysteroid (17 beta) dehydrogenase 8; Ke-6; KE6; mouse homolog of; Protein Ke6; Really interesting new gene 2 protein; RING2; SDR30C1; Short chain dehydrogenase/reductase family 30C member 1; Testosterone 17 beta dehydrogenase 8; Testosterone 17-beta-dehydrogenase 8; anti-HSD17B8 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
HSD17B8 antibody detects endogenous levels of total HSD17B8
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
261
Applicable Applications for anti-HSD17B8 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human HSD17B8
Subcellular Location
Mitochondrion
Tissue Specificity
Highly expressed in placenta, liver and pancreas, lower in the skeletal muscle and kidney. Widely expressed.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of HSD17B8 using A549 whole cell lysates)
Western Blot (WB)
(Western blot analysis of HSD17B8 using A549 whole cell lysates)
Related Product Information for anti-HSD17B8 antibody
Function: NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (PubMed:17978863). The heterotetramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis (PubMed:19571038, PubMed:25203508). Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD (PubMed:25203508).
Subunit Structure: Heterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
Subunit Structure: Heterotetramer with CBR4; contains two molecules of HSD17B8 and CBR4.
Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 27 kDa
Predicted: 27 kDa
Predicted: 27 kDa
NCBI Official Full Name
estradiol 17-beta-dehydrogenase 8
NCBI Official Synonym Full Names
hydroxysteroid 17-beta dehydrogenase 8
NCBI Official Symbol
HSD17B8
NCBI Official Synonym Symbols
KE6; FABG; HKE6; FABGL; RING2; H2-KE6; SDR30C1; D6S2245E; dJ1033B10.9
NCBI Protein Information
estradiol 17-beta-dehydrogenase 8
UniProt Protein Name
Estradiol 17-beta-dehydrogenase 8
Protein Family
UniProt Gene Name
HSD17B8
UniProt Synonym Gene Names
FABGL; HKE6; RING2; SDR30C1; 17-beta-HSD 8; KAR alpha subunit1 PublicationManual assertion based on opinion iniRef.12; Ke-6
NCBI Description
In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. The protein encoded by this gene is similar to Ke6 and is a member of the short-chain dehydrogenase superfamily. An alternatively spliced transcript of this gene has been detected, but the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008]
Uniprot Description
NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol. Has very low activity towards testosterone (PubMed:17978863). The heterotetramer with CBR4 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity, and thereby plays a role in mitochondrial fatty acid biosynthesis (PubMed:19571038, PubMed:25203508). Within the heterotetramer, HSD17B8 binds NADH; CBR4 binds NADPD (PubMed:25203508).
Research Articles on HSD17B8
Similar Products
Product Notes
The HSD17B8 hsd17b8 (Catalog #AAA9609823) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HSD17B8 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSD17B8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the HSD17B8 hsd17b8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSD17B8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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