Testing Data
anti-Human, Mouse KIF2C Antibody | anti-KIF2C antibody
KIF2C (Phospho-Ser95) Antibody
Gene Names
KIF2C; MCAK; CT139; KNSL6
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Synonyms
KIF2C; KIF2C (Phospho-Ser95) Antibody; KF2C; KNSL6; Kinesin-like protein 6; Kinesin-like protein KIF2C; Mitotic centromere-associated kinesin; anti-KIF2C antibody
Reactivity
Human, Mouse
Specificity
KIF2C (Phospho-Ser95) antibody detects endogenous levels of KIF2C only when phosphorylated at serine 95.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
684
Applicable Applications for anti-KIF2C antibody
Western Blot (WB), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:1000
IF: 1:100~1:500
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human KIF2C around the phosphorylation site of serine 95 (R-R-SP-V-N).
All Sites
Human: Ser95
Mouse: Ser92
Mouse: Ser92
Preparation and Storage
Store at-20 degree C for 1 year.
Testing Data
Testing Data
Western Blot (WB)
(P-peptide-+ Western blot analysis of extracts from HeLa cells, treated with TNF (10ng/ml, 30mins), using KIF2C (Phospho-Ser95) antibody. Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) antibody.)
Western Blot (WB)
(P-peptide-+ Western blot analysis of extracts from HeLa cells, treated with TNF (10ng/ml, 30mins), using KIF2C (Phospho-Ser95) antibody. Immunofluorescence analysis of A549 cells, using KIF2C (Phospho-Ser95) antibody.)
Product Categories/Family for anti-KIF2C antibody
References
Kim I.-G., Biochim. Biophys. Acta 1359:181-186(1997). Cheng L.J.; Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases. The MGC Project Team; Genome Res. 14:2121-2127(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
75,561 Da
NCBI Official Full Name
kinesin-like protein KIF2C isoform 2
NCBI Official Synonym Full Names
kinesin family member 2C
NCBI Official Symbol
KIF2C
NCBI Official Synonym Symbols
MCAK; CT139; KNSL6
NCBI Protein Information
kinesin-like protein KIF2C
UniProt Protein Name
Kinesin-like protein KIF2C
Protein Family
UniProt Gene Name
KIF2C
UniProt Synonym Gene Names
KNSL6; MCAK
NCBI Description
This gene encodes a kinesin-like protein that functions as a microtubule-dependent molecular motor. The encoded protein can depolymerize microtubules at the plus end, thereby promoting mitotic chromosome segregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
In complex with KIF18B, constitutes the major microtubule plus-end depolymerizing activity in mitotic cells (PubMed:21820309). Regulates the turnover of microtubules at the kinetochore and functions in chromosome segregation during mitosis (PubMed:19060894). Plays a role in chromosome congression and is required for the lateral to end-on conversion of the chromosome-microtubule attachment (PubMed:23891108).
Research Articles on KIF2C
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Product Notes
The KIF2C kif2c (Catalog #AAA859545) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The KIF2C (Phospho-Ser95) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIF2C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), ELISA (EIA). WB: 1:500~1:1000 IF: 1:100~1:500 ELISA: 1:1000. Researchers should empirically determine the suitability of the KIF2C kif2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF2C, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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