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Western Blot (WB) (Western blot analysis of extracts from RAW264.7 cells, treated with G-CSF (25ng/ml, 15mins), using IL3R (Ab-593) antibody.)

anti-Human, Mouse IL3R Antibody | anti-IL3R antibody

IL3R (Ab-593) Antibody

Gene Names
IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Synonyms
IL3R; IL3R (Ab-593) Antibody; Interleukin-3 receptor alpha chain; IL-3R-alpha; CD123 antigen; IL3RA; anti-IL3R antibody
Ordering
For Research Use Only!
Reactivity
Human, Mouse
Specificity
IL3R (Ab-593) antibody detects endogenous levels of total IL3R protein.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Sequence Length
300
Applicable Applications for anti-IL3R antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
IHC: 1:50~1:100
ELISA: 1:1000
Immunogen
The antiserum was produced against synthesized non-phosphopeptide derived from human IL3R around the phosphorylation site of tyrosine 593 (G-P-Y-L-G).
Preparation and Storage
Store at-20 degree C for 1 year

Western Blot (WB)

(Western blot analysis of extracts from RAW264.7 cells, treated with G-CSF (25ng/ml, 15mins), using IL3R (Ab-593) antibody.)

Western Blot (WB) (Western blot analysis of extracts from RAW264.7 cells, treated with G-CSF (25ng/ml, 15mins), using IL3R (Ab-593) antibody.)
References
Michelle M. Le Beau, Blood, Aug 2003; 102: 1072-1074. Elena Cattaneo, J. Biol. Chem., Sep 1996; 271: 23374. Patricia McLoughlin, J. Biol. Chem., Sep 2002; 277: 37045-37053. Louise Edvardsson, Blood, Nov 2004; 104: 4120.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34,466 Da
NCBI Official Full Name
interleukin-3 receptor subunit alpha isoform 2
NCBI Official Synonym Full Names
interleukin 3 receptor subunit alpha
NCBI Official Symbol
IL3RA
NCBI Official Synonym Symbols
IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
NCBI Protein Information
interleukin-3 receptor subunit alpha
UniProt Protein Name
Interleukin-3 receptor subunit alpha
UniProt Gene Name
IL3RA
UniProt Synonym Gene Names
IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA

NCBI Description

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]

Uniprot Description

This is a receptor for interleukin-3.

Research Articles on IL3R

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Product Notes

The IL3R il3ra (Catalog #AAA8509420) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The IL3R (Ab-593) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL3R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:1000. Researchers should empirically determine the suitability of the IL3R il3ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL3R, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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