anti-Human, Mouse hnRNP K Antibody | anti-hnRNP K antibody

hnRNP K (Ab-284) Antibody

Cat. Num. AAA8509730

• Gene Names: HNRNPK; AUKS; CSBP; TUNP; HNRPK
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: hnRNP K; hnRNP K (Ab-284) Antibody; 65 kDa phosphoprotein; CSBP; DC-stretch binding protein; Heterogeneous nuclear ribonucleoprotein K; hnRNP-K; HNRNPK; HNRPK; Transformation upregulated nuclear protein; TUNP; anti-hnRNP K antibody

• Reactivity: Human, Mouse
• Specificity: hnRNP K (Ab-284) antibody detects endogenous levels of total hnRNP K protein.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 439

• Applicable Applications for anti-hnRNP K antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:40000
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human hnRNP K around the phosphorylation site of serine 284(D-M-SP-P-R).
• Preparation and Storage: Store at-20 degree C for 1 year.

Western Blot (WB)

(Western blot analysis of extracts from LOVO cells and RAW264.7 cells, using hnRNP K (Ab-284) antibody.)

References

Matunis M.J., Mol. Cell. Biol. 12:164-171(1992). Dejgaard K., J. Mol. Biol. 236:33-48(1994). The MGC Project Team; Genome Res. 14:2121-2127(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 970598247
• NCBI GeneID: 3190
• NCBI Accession #: NP_001305115.1
• NCBI GenBank Nucleotide #: NM_001318186.1
• UniProt Accession #: P61978; Q07244; Q15671; Q59F98; Q5T6W4; Q60577; Q6IBN1; Q922Y7; Q96J62
• Molecular Weight: 48,562 Da
• NCBI Official Full Name: heterogeneous nuclear ribonucleoprotein K isoform c
• NCBI Official Synonym Full Names: heterogeneous nuclear ribonucleoprotein K
• NCBI Official Symbol: HNRNPK
• NCBI Official Synonym Symbols: AUKS; CSBP; TUNP; HNRPK
• NCBI Protein Information: heterogeneous nuclear ribonucleoprotein K
• UniProt Protein Name: Heterogeneous nuclear ribonucleoprotein K
• UniProt Gene Name: HNRNPK
• UniProt Synonym Gene Names: HNRPK; hnRNP K; TUNP

NCBI Description

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene is located in the nucleoplasm and has three repeats of KH domains that binds to RNAs. It is distinct among other hnRNP proteins in its binding preference; it binds tenaciously to poly(C). This protein is also thought to have a role during cell cycle progession. Several alternatively spliced transcript variants have been described for this gene, however, not all of them are fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. Plays an important role in p53/TP53 response to DNA damage, acting at the level of both transcription activation and repression. When sumoylated, acts as a transcriptional coactivator of p53/TP53, playing a role in p21/CDKN1A and 14-3-3 sigma/SFN induction (). As far as transcription repression is concerned, acts by interacting with long intergenic RNA p21 (lincRNA-p21), a non-coding RNA induced by p53/TP53. This interaction is necessary for the induction of apoptosis, but not cell cycle arrest.