anti-Human, Mouse DAPK2 Antibody | anti-DAPK2 antibody

DAPK2 (Phospho-Ser318) Antibody

Cat. Num. AAA8509639

• Gene Names: DAPK2; DRP1; DRP-1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Immunofluorescence, ELISA
• Purity: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Synonyms: DAPK2; DAPK2 (Phospho-Ser318) Antibody; DAP-kinase related protein 1; DRP-1; EC 2.7.11.1; kinase DAPK2; anti-DAPK2 antibody

• Reactivity: Human, Mouse
• Specificity: DAPK2 (Phospho-Ser318) antibody detects endogenous levels of DAPK2 only when phosphorylated at serine 318.
• Purity/Purification: Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Form/Format: In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 370

• Applicable Applications for anti-DAPK2 antibody: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; IF: 1:100-1:500; ELISA: 1:5000
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human DAPK2 around the phosphorylation site of serine 318 (K-L-SP-F-S).
• All Sites: Human: Ser318; Mouse: Ser251
• Preparation and Storage: Store at-20 degree C for 1 year.

Testing Data

(P-peptide-+)

Immunohistochemistry (IHC)

(P-peptide-+ Immunohistochemistry analysis of paraffin-embedded human skeletal muscle tissue using DAPK2 (Phospho-Ser318) antibody. Immunofluorescence analysis of COS7 cells, using DAPK2 (Phospho-Ser318) antibody.)

References

Kawai T., Oncogene 18:3471-3480(1999). Inbal B., Mol. Cell. Biol. 20:1044-1054(2000). Shani G., EMBO J. 20:1099-1113(2001). Olsen J.V., Cell 127:635-648(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 14670383
• NCBI GeneID: 23604
• NCBI Accession #: NP_055141.2
• NCBI GenBank Nucleotide #: NM_014326.3
• UniProt Accession #: Q9UIK4; O75892; Q24JS1; E9JGM7
• Molecular Weight: 55,931 Da
• NCBI Official Full Name: death-associated protein kinase 2
• NCBI Official Synonym Full Names: death associated protein kinase 2
• NCBI Official Symbol: DAPK2
• NCBI Official Synonym Symbols: DRP1; DRP-1
• NCBI Protein Information: death-associated protein kinase 2
• UniProt Protein Name: Death-associated protein kinase 2
• Protein Family: Death-associated protein kinase
• UniProt Gene Name: DAPK2
• UniProt Synonym Gene Names: DAP kinase 2; DRP-1

NCBI Description

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq, Jul 2008]

Uniprot Description

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed:17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed:24163421).