SDS-Page
(SDS-PAGE of 70kDa Hsp70 protein (SPR-108).)
Hsp70 Active Protein | HSPA1A active protein
Recombinant Human HSP70, native sequence
Gene Names
HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
Reactivity
Human
Applications
Western Blot, ELISA
Synonyms
Hsp70; Recombinant Human HSP70; native sequence; Hsp70 1; Hsp70 2; Hsp70.1; Hsp72; Hsp73; HSPA1; HSPA1A; HSPA1B; HSPA1A active protein
Host
Human
Reactivity
Human
Form/Format
Multi-Step Chromatography
Concentration
0.88mg/mL (varies by lot)
Sequence Length
641
Applicable Applications for HSPA1A active protein
ATPase Assay, Western Blot control (WB), Binding Assays, ELISA (EIA) reference standard, Lipid Interaction Assays, Chaperone assays
Certificate of Analysis
This product has been certified >95% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.0uM phosphate liberated/hr/ug protein in a 200ul reaction at 37 degree C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Conjugate Tag
No tags
Expression System
E Coli
Storage Buffer
50mM Tris/HCl pH7.5, 2.5mM Bme, 0.15M NaCl, and 10% glycerol
Preparation and Storage
-20 degree C
SDS-Page
(SDS-PAGE of 70kDa Hsp70 protein (SPR-108).)
SDS-Page
(SDS-PAGE of 70kDa Hsp70 protein (SPR-108).)
Related Product Information for HSPA1A active protein
Background Info: Molecular weight of approximately 70kDa
ATPase active, native
Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
ATPase active, native
Scientific Background: Hsp70 genes encode abundant heat-inducible 70-kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (2). The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.
Product Categories/Family for HSPA1A active protein
References
1. Zho, J. (1998). Cell 94: 471-480. 2. Boorstein, W. R., Ziegelhoffer, T. & Craig, E. A. (1993), J. Mol. Evol.38 (1) 1-17. 3. Rothman, J. (1989), Cell 59, 591 -601. 4. DeLuca-Flaherty et al. (1990), Cell 62, 875-887. 5. Bork, P., Sander, C. & Valencia, A. (1992), Proc. Nut1 Acad. Sci. USA 89, 7290-7294. 6. Fink, A.L. (1999) Physiol. Rev. 79: 425-449. 7. Smith, D.F., et al., (1993) Mol. Cell. Biol. 13(2):869-876. 8. Prapapanich, V., et al., (1996) Mol. Cell. Biol. 16(11):6200-6207. 9. Fernandez-Funez et al., (2000) Nature 408(6808):101-106.
1. Ishibashi, Y., Kato, H., Asahi, Y., Suqita, T. and Nishikawa, A. (2009). Identification of the major allergen of Malassezia globosa relevant for atopic dermatitis. Journal of Dermatological Science. 55 (3), 185-192. doi:10.1016/j.jdermsci.2009.05.005
1. Ishibashi, Y., Kato, H., Asahi, Y., Suqita, T. and Nishikawa, A. (2009). Identification of the major allergen of Malassezia globosa relevant for atopic dermatitis. Journal of Dermatological Science. 55 (3), 185-192. doi:10.1016/j.jdermsci.2009.05.005
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
70kD
NCBI Official Full Name
heat shock 70 kDa protein 1A/1B
NCBI Official Synonym Full Names
heat shock 70kDa protein 1A
NCBI Official Symbol
HSPA1A
NCBI Official Synonym Symbols
HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103
NCBI Protein Information
heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; epididymis secretory protein Li 103; dnaK-type molecular chaperone HSP70-1
UniProt Protein Name
Heat shock 70 kDa protein 1A/1B
Protein Family
UniProt Gene Name
HSPA1A
UniProt Synonym Gene Names
HSPA1; HSX70
HSPA1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2
HSPA1B; HSP70-1/HSP70-2; HSP70.1/HSP70.2
UniProt Entry Name
HSP71_HUMAN
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Product Notes
The HSPA1A hspa1a (Catalog #AAA806938) is an Active Protein produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Human HSP70, native sequence reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Hsp70 can be used in a range of immunoassay formats including, but not limited to, ATPase Assay, Western Blot control (WB), Binding Assays, ELISA (EIA) reference standard, Lipid Interaction Assays, Chaperone assays. Researchers should empirically determine the suitability of the HSPA1A hspa1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hsp70, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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