Creatine Kinase MM (CK-MM) >95% Pure Active Protein | CKM active protein

Human Creatine Kinase-MM

Cat. Num. AAA318120

• Gene Names: CKM; CKMM; M-CK
• Applications: Specific methodologies have not been tested using this product.
• Purity: 1 CK band by agarose gel isoenzyme electrophoresis. Molecular weight of the MM fraction is unknown.
• Synonyms: Creatine Kinase MM (CK-MM) >95% Pure; Human Creatine Kinase-MM; Purified Creatine Kinase MM (CKMM) isoenzyme fraction; CKM active protein

• Host: Human heart
• Purity/Purification: 1 CK band by agarose gel isoenzyme electrophoresis. Molecular weight of the MM fraction is unknown.
• Form/Format: Purified, Lyophilized.
• Concentration: 0.32 mg/mL protein/vial(Coomasie)( prior to lyophilization). (varies by lot)
• Sequence Length: 381

• Applicable Applications for CKM active protein: Specific methodologies have not been tested using this product.
• Activity: Minimum 100 U/vial @37°C (measured on Dimension); Measured at 340 nm as one equimolar amount of NADH produced by coupled reaction.
• Specific Activity: 530 Units/mg protein.; One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37°C.
• Inactivation: Not applicable
• Buffer: Lyophilized from 40mM Tris-Cl, 1mM EDTA, 10mM N-Acetyl cysteine, pH 7.5
• Preservative: No
• Preparation and Storage: Store at -20°C . Avoid multiple freeze/thaw cycles.

Product Categories/Family for CKM active protein

Purified Cardiac Markers

NCBI and Uniprot Product Information

• NCBI GI #: 180588
• NCBI GeneID: 1158
• UniProt Accession #: P06732; Q96QL9
• NCBI Official Full Name: creatine kinase
• NCBI Official Synonym Full Names: creatine kinase, muscle
• NCBI Official Symbol: CKM
• NCBI Official Synonym Symbols: CKMM; M-CK
• NCBI Protein Information: creatine kinase M-type; creatine kinase-M; creatine kinase M chain
• UniProt Protein Name: Creatine kinase M-type
• Protein Family: Creatine kinase
• UniProt Gene Name: CKM
• UniProt Synonym Gene Names: CKMM
• UniProt Entry Name: KCRM_HUMAN

NCBI Description

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq]

Uniprot Description

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity: ATP + creatine = ADP + phosphocreatine.

Subunit structure: Dimer of identical or non-identical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.

Subcellular location: Cytoplasm.

Sequence similarities: Belongs to the ATP:guanido phosphotransferase family.Contains 1 phosphagen kinase C-terminal domain.Contains 1 phosphagen kinase N-terminal domain.