Human Zinc finger MYND domain-containing protein 19 (ZMYND19) ELISA Kit | ZMYND19 elisa kit

Human Zinc finger MYND domain-containing protein 19 (ZMYND19) ELISA Kit

Cat. Num. AAA286578

• Gene Names: ZMYND19; MIZIP
• Reactivity: Human
• Synonyms: Zinc finger MYND domain-containing protein 19 (ZMYND19); Human Zinc finger MYND domain-containing protein 19 (ZMYND19) ELISA Kit; MIZIP; RP11-48C7.4; melanin-concentrating hormone receptor 1 interacting zinc-finger protein; zinc finger; MYND domain containing 19; ZMYND19 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ZMYND19. No significant cross-reactivity or interference between Human ZMYND19 and analogues was observed.
• Sequence Length: 227

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ZMYND19 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate ZMYND19 in samples. An antibody specific for ZMYND19 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ZMYND19 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZMYND19 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZMYND19 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: The deduced 227-amino acid protein contains a C-terminal zinc finger motif that shares a high degree of amino acid identity to the zinc finger domains of myeloid translocation protein-8 (CBFA2T1), Nervy, DEAF1 (MYND) proteins. ZMYND19 contains multiple putative phosphorylation sites. The deduced human, mouse, and rat ZMYND19 proteins share 100% amino acid identity. Immunocytochemistry of transfected HEK293 cells localized ZMYND19 to the cytoplasm and MCHR1 to the plasma membrane; however, ZMYND19 colocalized to the plasma membrane when cotransfected with MCHR1. Northern blot analysis of human tissues detected a 1.5-kb transcript in all areas of the brain, especially cerebellum, and in heart, liver, skeletal muscle, kidney, testis, placenta, and stomach.

NCBI and Uniprot Product Information

• NCBI GI #: 19923997
• NCBI GeneID: 116225
• NCBI Accession #: NP_612471.1
• NCBI GenBank Nucleotide #: NM_138462.2
• UniProt Accession #: Q96E35; Q5T366
• Molecular Weight: 26,433 Da
• NCBI Official Full Name: zinc finger MYND domain-containing protein 19
• NCBI Official Synonym Full Names: zinc finger MYND-type containing 19
• NCBI Official Symbol: ZMYND19
• NCBI Official Synonym Symbols: MIZIP
• NCBI Protein Information: zinc finger MYND domain-containing protein 19
• UniProt Protein Name: Zinc finger MYND domain-containing protein 19
• Protein Family: Zinc finger MYND domain-containing protein
• UniProt Gene Name: ZMYND19
• UniProt Synonym Gene Names: MIZIP; MCH-R1-interacting zinc finger protein

NCBI Description

ZMYND19 is a MYND zinc finger domain-containing protein that binds to the C terminus of melanin-concentrating hormone receptor-1 (MCHR1; MIM 601751) (Bachner et al., 2002 [PubMed 12208518]), and to the N termini of alpha-tubulin (TUBA1; MIM 191110), and beta-tubulin (TUBB; MIM 191130) (Francke et al., 2005 [PubMed 16039987]).[supplied by OMIM, Mar 2008]

Uniprot Description

May be involved as a regulatory molecule in GPR24/MCH-R1 signaling.