Human Yorkie homolog (YAP1) ELISA Kit | YAP1 elisa kit

Human Yorkie homolog (YAP1) ELISA Kit

Cat. Num. AAA286628

• Gene Names: YAP1; YAP; YKI; COB1; YAP2; YAP65
• Reactivity: Human
• Synonyms: Yorkie homolog (YAP1); Human Yorkie homolog (YAP1) ELISA Kit; YAP; YAP2; YAP65; YKI; yes-associated protein 2; YAP1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human YAP1. No significant cross-reactivity or interference between Human YAP1 and analogues was observed.
• Sequence Length: 504

• Assay Type: Quantitive Sandwich
• Samples: Serum, Plasma, Other Biological Fluids
• Detection Wavelength: 450 nm
• Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
• Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
• CV (%): = SD/meanX100
• Intra-Assay: CV
• Inter-Assay: CV
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for YAP1 elisa kit

Description: This assay employs a two-site sandwich ELISA to quantitate YAP1 in samples. An antibody specific for YAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any YAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Overview: YAP1 contains a WW domain that is found in various structural, regulatory and signaling molecules in yeast, nematode, and mammals, and may be involved in protein-protein interaction.
The human and mouse cDNAs encode deduced 493- and 531-amino acid proteins, respectively, with significant sequence similarity to the chicken protein. All 3 contain a putative protein module of 38 amino acids that is found in various structural, regulatory, and signaling molecules in yeast, nematode, and mammals and may be involved in protein-protein interaction. The mouse protein contains an imperfect repeat of the module. Because one of the prominent features of the sequence motif is 2 tryptophans, it has been designated the WW domain.

NCBI and Uniprot Product Information

• NCBI GI #: 194306653
• NCBI GeneID: 10413
• NCBI Accession #: NP_001123617.1
• NCBI GenBank Nucleotide #: NM_001130145.2
• UniProt Accession #: P46937; Q7Z574; B4DTY1; B7ZA01; E3WEB5; E3WEB6; E9PRV2; F5H202; K0KQ18; K0KYZ8; K0L195; K0L1G3
• Molecular Weight: 54,942 Da
• NCBI Official Full Name: transcriptional coactivator YAP1 isoform 1
• NCBI Official Synonym Full Names: Yes associated protein 1
• NCBI Official Symbol: YAP1
• NCBI Official Synonym Symbols: YAP; YKI; COB1; YAP2; YAP65
• NCBI Protein Information: transcriptional coactivator YAP1
• UniProt Protein Name: Transcriptional coactivator YAP1
• Protein Family: Transcriptional coactivator
• UniProt Gene Name: YAP1
• UniProt Synonym Gene Names: YAP65; Yes-associated protein 1

NCBI Description

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Uniprot Description

Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637). The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288). Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702). Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288). The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).