Human Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit | WASF2 elisa kit

Human Wiskott Aldrich Syndrome Protein Family, Member 2 (WASF2) ELISA Kit

Cat. Num. AAA451757

• Gene Names: WASF2; IMD2; SCAR2; WASF4; WAVE2; dJ393P12.2
• Reactivity: Human
• Synonyms: Wiskott Aldrich Syndrome Protein Family; Member 2 (WASF2); Human Wiskott Aldrich Syndrome Protein Family; Member 2 (WASF2) ELISA Kit; SCAR2; WAVE2; Verprolin homology domain-containing protein 2; WASF2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WASF2. No significant cross-reactivity or interference between WASF2 and analogues was observed.
• Sequence Length: 281

• Samples: Tissue homogenates, cell lysates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.106ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WASF2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WASF2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WASF2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WASF2 in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WASF2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to WASF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WASF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WASF2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 319655728
• NCBI GeneID: 10163
• NCBI Accession #: NP_001188333.1
• NCBI GenBank Nucleotide #: NM_001201404.2
• UniProt Accession #: Q9Y6W5; O60794; Q9UDY7; B4DZN0
• Molecular Weight: 31,979 Da
• NCBI Official Full Name: wiskott-Aldrich syndrome protein family member 2 isoform 2
• NCBI Official Synonym Full Names: WAS protein family member 2
• NCBI Official Symbol: WASF2
• NCBI Official Synonym Symbols: IMD2; SCAR2; WASF4; WAVE2; dJ393P12.2
• NCBI Protein Information: wiskott-Aldrich syndrome protein family member 2
• UniProt Protein Name: Wiskott-Aldrich syndrome protein family member 2
• Protein Family: Wiskott-Aldrich syndrome protein family
• UniProt Gene Name: WASF2
• UniProt Synonym Gene Names: WAVE2; WASP family protein member 2

NCBI Description

This gene encodes a member of the Wiskott-Aldrich syndrome protein family. The gene product is a protein that forms a multiprotein complex that links receptor kinases and actin. Binding to actin occurs through a C-terminal verprolin homology domain in all family members. The multiprotein complex serves to tranduce signals that involve changes in cell shape, motility or function. The published map location (PMID:10381382) has been changed based on recent genomic sequence comparisons, which indicate that the expressed gene is located on chromosome 1, and a pseudogene may be located on chromosome X. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]

Uniprot Description

Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.