Typical Testing Data/Standard Curve (for reference only)
Human WD repeat containing protein 1 (WDR1) ELISA Kit | WDR1 elisa kit
Human WD repeat containing protein 1 (WDR1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: WDR1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-WDR1 antibody and an WDR1-HRP conjugate. The assay sample and buffer are incubated together with WDR1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WDR1 concentration since WDR1 from samples and WDR1-HRP conjugate compete for the anti-WDR1 antibody binding site. Since the number of sites is limited, as more sites are occupied by WDR1 from the sample, fewer sites are left to bind WDR1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WDR1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
WDR1: a WD40 repeat cytoskeletal protein that induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. Three alternatively spliced human isoforms have been described.
Protein type: Actin-binding
Chromosomal Location of Human Ortholog: 4p16.1
Cellular Component: cell projection; cytoplasm; extracellular region; actin filament; podosome; cytosol; cell junction
Molecular Function: actin binding
Biological Process: platelet activation; platelet degranulation; sensory perception of sound; regulation of oligodendrocyte differentiation; blood coagulation
Research Articles on WDR1
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Product Notes
The Human WDR1 wdr1 (Catalog #AAA7251079) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7251079 ELISA Kit recognizes Human WDR1. It is sometimes possible for the material contained within the vial of "WD repeat containing protein 1 (WDR1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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