Human WAP four disulfide core domain protein 1 (WFDC1) ELISA Kit | WFDC1 elisa kit

Human WAP four disulfide core domain protein 1 (WFDC1) ELISA Kit

Cat. Num. AAA7244225

• Gene Names: WFDC1; PS20
• Reactivity: Human
• Synonyms: WAP four disulfide core domain protein 1 (WFDC1); Human WAP four disulfide core domain protein 1 (WFDC1) ELISA Kit; WFDC1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WFDC1. No significant cross-reactivity or interference between WFDC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between WFDC1 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WFDC1 elisa kit

Intended Uses: This WFDC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human WFDC1. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: WFDC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-WFDC1 antibody and an WFDC1-HRP conjugate. The assay sample and buffer are incubated together with WFDC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the WFDC1 concentration since WFDC1 from samples and WFDC1-HRP conjugate compete for the anti-WFDC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by WFDC1 from the sample, fewer sites are left to bind WFDC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The WFDC1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for WFDC1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 18379353
• NCBI GeneID: 58189
• NCBI Accession #: NP_067020.2
• NCBI GenBank Nucleotide #: NM_021197.3
• Molecular Weight: 23,977 Da
• NCBI Official Full Name: WAP four-disulfide core domain protein 1 isoform 1
• NCBI Official Synonym Full Names: WAP four-disulfide core domain 1
• NCBI Official Symbol: WFDC1
• NCBI Official Synonym Symbols: PS20
• NCBI Protein Information: WAP four-disulfide core domain protein 1; ps20 growth inhibitor; prostate stromal protein ps20; WAP four-disulfide core domain 1 homolog
• UniProt Protein Name: WAP four-disulfide core domain protein 1
• Protein Family: WAP four-disulfide core domain protein
• UniProt Gene Name: WFDC1
• UniProt Synonym Gene Names: PS20
• UniProt Entry Name: WFDC1_HUMAN

NCBI Description

This gene encodes a member of the WAP-type four disulfide core domain family. The WAP-type four-disulfide core domain contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is mapped to chromosome 16q24, an area of frequent loss of heterozygosity in cancers, including prostate, breast and hepatocellular cancers and Wilms' tumor. This gene is downregulated in many cancer types and may be involved in the inhibition of cell proliferation. The encoded protein may also play a role in the susceptibility of certain CD4 memory T cells to human immunodeficiency virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]

Uniprot Description

WFDC1: Has growth inhibitory activity.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity

Biological Process: response to drug; negative regulation of cell growth; response to estradiol stimulus; negative regulation of epithelial cell proliferation