Human WDR1 (WD repeat-containing protein 1) ELISA Kit | WDR1 elisa kit

Human WDR1 (WD repeat-containing protein 1) ELISA Kit

Cat. Num. AAA7612212

• Gene Names: WDR1; AIP1; NORI-1; HEL-S-52
• Reactivity: Human
• Synonyms: WDR1 (WD repeat-containing protein 1); Human WDR1 (WD repeat-containing protein 1) ELISA Kit; WD repeat-containing protein 1; Actin-interacting protein 1; AIP1; NORI-1; WDR1; WDR1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WDR1. No significant cross-reactivity or interference between WDR1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WDR1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 53729352
• NCBI GeneID: 9948
• NCBI Accession #: NP_005103.2
• NCBI GenBank Nucleotide #: NM_005112.4
• UniProt Accession #: O75083; O75313; Q8N6E5; Q9UG05; Q9UG78; Q9UQE0; A8K6E9; A8MPU4
• Molecular Weight: 50,694 Da
• NCBI Official Full Name: WD repeat-containing protein 1 isoform 2
• NCBI Official Synonym Full Names: WD repeat domain 1
• NCBI Official Symbol: WDR1
• NCBI Official Synonym Symbols: AIP1; NORI-1; HEL-S-52
• NCBI Protein Information: WD repeat-containing protein 1
• UniProt Protein Name: WD repeat-containing protein 1
• Protein Family: WD repeat-containing protein
• UniProt Gene Name: WDR1
• UniProt Synonym Gene Names: AIP1
• UniProt Entry Name: WDR1_HUMAN

NCBI Description

This gene encodes a protein containing 9 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, mostly including a trp-asp at the C-terminal end. WD domains are involved in protein-protein interactions. The encoded protein may help induce the disassembly of actin filaments. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

WDR1: a WD40 repeat cytoskeletal protein that induces disassembly of actin filaments in conjunction with ADF/cofilin family proteins. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly. Three alternatively spliced human isoforms have been described.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 4p16.1

Cellular Component: cytoplasm; cytosol; extracellular region

Biological Process: platelet degranulation; sensory perception of sound